Akdag Mehmet Zulkuf, Dasdag Suleyman, Canturk Fazile, Karabulut Derya, Caner Yusuf, Adalier Nur
Department of Biophysics, Medical Faculty of Dicle University, 21280 Diyarbakir, Turkey.
Department of Biophysics, Medical Faculty of Istanbul Medeniyet University, Istanbul, Turkey.
J Chem Neuroanat. 2016 Sep;75(Pt B):116-22. doi: 10.1016/j.jchemneu.2016.01.003. Epub 2016 Jan 8.
Wireless internet (Wi-Fi) providers have become essential in our daily lives, as wireless technology is evolving at a dizzying pace. Although there are different frequency generators, one of the most commonly used Wi-Fi devices are 2.4GHz frequency generators. These devices are heavily used in all areas of life but the effect of radiofrequency (RF) radiation emission on users is generally ignored. Yet, an increasing share of the public expresses concern on this issue. Therefore, this study intends to respond to the growing public concern. The purpose of this study is to reveal whether long term exposure of 2.4GHz frequency RF radiation will cause DNA damage of different tissues such as brain, kidney, liver, and skin tissue and testicular tissues of rats. The study was conducted on 16 adult male Wistar-Albino rats. The rats in the experimental group (n=8) were exposed to 2.4GHz frequency radiation for over a year. The rats in the sham control group (n=8) were subjected to the same experimental conditions except the Wi-Fi generator was turned off. After the exposure period was complete the possible DNA damage on the rat's brain, liver, kidney, skin, and testicular tissues was detected through the single cell gel electrophoresis assay (comet) method. The amount of DNA damage was measured as percentage tail DNA value. Based on the DNA damage results determined by the single cell gel electrophoresis (Comet) method, it was found that the% tail DNA values of the brain, kidney, liver, and skin tissues of the rats in the experimental group increased more than those in the control group. The increase of the DNA damage in all tissues was not significant (p>0.05). However the increase of the DNA damage in rat testes tissue was significant (p<0.01). In conclusion, long-term exposure to 2.4GHz RF radiation (Wi-Fi) does not cause DNA damage of the organs investigated in this study except testes. The results of this study indicated that testes are more sensitive organ to RF radiation.
随着无线技术以惊人的速度发展，无线互联网（Wi-Fi）提供商在我们的日常生活中变得至关重要。尽管有不同的频率发生器，但最常用的Wi-Fi设备之一是2.4GHz频率发生器。这些设备在生活的各个领域都被大量使用，但射频（RF）辐射发射对用户的影响通常被忽视。然而，越来越多的公众对这个问题表示关注。因此，本研究旨在回应公众日益增长的关注。本研究的目的是揭示长期暴露于2.4GHz频率的射频辐射是否会导致大鼠的大脑、肾脏、肝脏、皮肤组织和睾丸组织等不同组织的DNA损伤。该研究对16只成年雄性Wistar-白化大鼠进行。实验组（n=8）的大鼠暴露于2.4GHz频率辐射一年以上。假对照组（n=8）的大鼠除关闭Wi-Fi发生器外，接受相同的实验条件。暴露期结束后，通过单细胞凝胶电泳分析（彗星）法检测大鼠大脑、肝脏、肾脏、皮肤和睾丸组织中可能的DNA损伤。DNA损伤量以尾DNA值百分比来衡量。根据单细胞凝胶电泳（彗星）法测定的DNA损伤结果，发现实验组大鼠大脑、肾脏、肝脏和皮肤组织的尾DNA值百分比增加幅度大于对照组。所有组织中DNA损伤的增加均无统计学意义（p>0.05）。然而，大鼠睾丸组织中DNA损伤的增加具有统计学意义（p<0.01）。总之，长期暴露于2.4GHz射频辐射（Wi-Fi）除睾丸外，不会导致本研究中所调查器官的DNA损伤。本研究结果表明，睾丸是对射频辐射更敏感的器官。
