Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.
Food Chem. 2016 May 15;199:364-71. doi: 10.1016/j.foodchem.2015.12.005. Epub 2015 Dec 8.
Enrichment of omega-3 fatty acids in cod liver oil via alternate operation of solvent winterization and enzymatic interesterification was attempted. Variables including separation method, solvent, oil concentration, time and temperature were optimized for the winterization. Meanwhile, Novozyme 435, Lipozyme RM IM and Lipozyme TL IM were screened for interesterification efficiency under different system air condition, time and temperature. In optimized method, alternate winterization (0.1g/mL oil/acetone, 24h, -80°C, precooled Büchner filtration) and interesterification (Lipozyme TL IM, N2 flow, 2.5h, 40°C) successfully doubled the omega-3 fatty acid content to 43.20 mol%. (1)H NMR was used to determine omega-3 fatty acid content, and GC-MS to characterize oil product, which mainly contained DHA (15.81 mol%) and EPA (20.23 mol%). The proposed method offers considerable efficiency and reduce production cost drastically. Oil produced thereof is with high quality and of particular importance for the development of omega-3 based active pharmaceutical ingredients.
通过溶剂冬化和酶酯交换的交替操作来富集鱼肝油中的ω-3 脂肪酸。针对冬化过程，优化了分离方法、溶剂、油浓度、时间和温度等变量。同时，筛选了 Novozyme 435、Lipozyme RM IM 和 Lipozyme TL IM 三种酶在不同体系空气条件、时间和温度下的酯交换效率。在优化的方法中，交替进行冬化（0.1g/mL 油/丙酮，24h，-80°C，预冷布氏漏斗过滤）和酯交换（Lipozyme TL IM，N2 流，2.5h，40°C），成功地将 ω-3 脂肪酸含量提高到 43.20mol%。（1）H NMR 用于测定 ω-3 脂肪酸含量，GC-MS 用于分析油产物，其主要成分是 DHA（15.81mol%）和 EPA（20.23mol%）。该方法效率高，可大幅降低生产成本。所生产的油质量高，对开发基于 ω-3 的活性药物成分具有重要意义。
