Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
Centre for the Study of Animal Sciences, CECA-ICETA, University of Porto, Porto, Portugal.
Vet Comp Oncol. 2017 Jun;15(2):655-666. doi: 10.1111/vco.12209. Epub 2016 Jan 17.
Human inflammatory breast cancer (IBC) and canine inflammatory mammary cancer (CIMC) are the most aggressive forms of mammary cancer. Current research aims to identify new therapeutic targets. Here, we investigated gene expression levels of biomarkers associated with the inflammatory microenvironment. A total of 32 formalin-fixed paraffin-embedded samples of canine mammary carcinoma (CIMC = 26; non-CIMC = 6) were used and their cDNA subjected to quantitative polymerase chain reaction (qPCR) to establish gene expression levels for mediators commonly implicated in linking carcinogenesis with inflammation. Gene expression differences between CIMC and non-CIMC types were obtained for cyclooxygenase 2 (COX-2) (P = 0.004), synuclein gamma (SNCG) (P = 0.006), tribbles 1 (P = 0.025), vascular endothelial growth factor (VEGF) (P = 0.017) and CSF1R (P = 0.045). Among these biomarkers correlations were found, particularly between SNCG and tribbles 1 (r = 0.512, P = 0.001). The efficient metastasis of CIMC is intimately linked to components in the tumour microenvironment. This study suggests that upregulation and correlation of SNCG and tribbles 1 deserves to be further explored.
人类炎性乳腺癌 (IBC) 和犬炎性乳腺肿瘤 (CIMC) 是最具侵袭性的乳腺肿瘤形式。目前的研究旨在确定新的治疗靶点。在这里，我们研究了与炎症微环境相关的生物标志物的基因表达水平。共使用了 32 个福尔马林固定石蜡包埋的犬乳腺肿瘤 (CIMC = 26;非 CIMC = 6) 样本，并对其 cDNA 进行了定量聚合酶链反应 (qPCR)，以确定与致癌作用与炎症相关的常见介质的基因表达水平。CIMC 和非 CIMC 类型之间的基因表达差异获得了环氧化酶 2 (COX-2) (P = 0.004)、突触核蛋白 γ (SNCG) (P = 0.006)、三叶草 1 (P = 0.025)、血管内皮生长因子 (VEGF) (P = 0.017) 和 CSF1R (P = 0.045)。在这些生物标志物中发现了相关性，特别是 SNCG 和 tribbles 1 之间的相关性 (r = 0.512, P = 0.001)。CIMC 的有效转移与肿瘤微环境中的成分密切相关。本研究表明，SNCG 和 tribbles 1 的上调和相关性值得进一步研究。
