Heunis T D J, Botes M, Dicks L M T
Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, 7600, Stellenbosch, South Africa.
Probiotics Antimicrob Proteins. 2010 Mar;2(1):46-51. doi: 10.1007/s12602-009-9024-9.
Plantaricin 423, produced by Lactobacillus plantarum 423, was encapsulated in nanofibers that were produced by the electrospinning of 18% (w/v) polyethylene oxide (200 000 Da). The average diameter of the nanofibers was 288 nm. Plantaricin 423 activity decreased from 51 200 AU/ml to 25 600 AU/ml and from 204 800 AU/ml to 51 200 AU/ml after electrospinning, as determined against Lactobacillus sakei DSM 20017 and Enterococcus faecium HKLHS, respectively. Cells of L. plantarum 423 encapsulated in nanofibers decreased from 2.3 × 10(10) cfu/ml before electrospinning to 4.7 × 10(8) cfu/ml thereafter. Cells entrapped in the nanofibers continued to produce plantaricin 423. This is the first report on the encapsulation of a bacteriocin and cells of L. plantarum in nanofibers. The method may be used to design a drug delivery system for bacteriocins and the encapsulation of probiotic lactic acid bacteria. The technology is currently being optimized.
植物乳杆菌423产生的植物乳杆菌素423被包裹在由18%（w/v）聚环氧乙烷（200 000 Da）静电纺丝制备的纳米纤维中。纳米纤维的平均直径为288纳米。分别以清酒乳杆菌DSM 20017和粪肠球菌HKLHS为检测对象，静电纺丝后植物乳杆菌素423的活性从51 200 AU/ml降至25 600 AU/ml，以及从204 800 AU/ml降至51 200 AU/ml。包裹在纳米纤维中的植物乳杆菌423细胞从静电纺丝前的2.3×10¹⁰ cfu/ml降至之后的4.7×10⁸ cfu/ml。包裹在纳米纤维中的细胞继续产生植物乳杆菌素423。这是关于将细菌素和植物乳杆菌细胞包裹在纳米纤维中的首次报道。该方法可用于设计细菌素的药物递送系统以及益生菌乳酸菌的包封。目前正在对该技术进行优化。
