Wu Deyao, Niu Xiaobing, Pan Huixing, Zhang Zichun, Zhou Yunfeng, Qu Ping, Zhou Jian
Department of Urology, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng City No. 1 People's Hospital, Yancheng, Jiangsu 224001, P.R. China.
Mol Med Rep. 2016 Mar;13(3):2287-92. doi: 10.3892/mmr.2016.4756. Epub 2016 Jan 11.
MicroRNA-497 (miR-497) has been reported to be downregulated in certain types of cancer, including breast, gastric, endometrial, colorectal and prostate cancer as well as hepatocellular and nasopharyngeal carcinoma. The present study aimed to investigate the underlying mechanism of the tumor suppressor function of miR‑497 in prostate cancer. Following transfection with miR‑497, the DU145 and PC‑3 prostate cancer cell lines were subjected to Transwell migration and invasion assays, western blot analysis and a luciferase assay. It was revealed that miRNA‑497 inhibited the migration and invasion of prostate cancer cells. In addition, is was indicated that miRNA‑497 directly targets hepatoma‑derived growth factor (HDGF) in prostate cancer cells. These results suggested that restoration of miR‑497 and the resulting downregulation of HDFG may represent a promising therapeutic strategy for prostate cancer.
据报道，微小RNA-497（miR-497）在某些类型的癌症中表达下调，包括乳腺癌、胃癌、子宫内膜癌、结直肠癌和前列腺癌以及肝细胞癌和鼻咽癌。本研究旨在探讨miR-497在前列腺癌中发挥肿瘤抑制功能的潜在机制。用miR-497转染后，对DU145和PC-3前列腺癌细胞系进行Transwell迁移和侵袭试验、蛋白质印迹分析和荧光素酶试验。结果显示，miRNA-497抑制前列腺癌细胞的迁移和侵袭。此外，有迹象表明miRNA-497在前列腺癌细胞中直接靶向肝癌衍生生长因子（HDGF）。这些结果表明，恢复miR-497并导致HDFG下调可能是一种有前景的前列腺癌治疗策略。
