Noh Soyoung, Gong Hye Yeon, Lee Hyun Jong, Koh Won-Gun
Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Korea.
Department of Chemical and Biological Engineering, Gachon University, 1342 Seongnamdaero, Gyeonggi-do 13120, Korea.
Materials (Basel). 2021 Jan 8;14(2):308. doi: 10.3390/ma14020308.
Hydrogel substrate-based micropatterns can be adjusted using the pattern shape and size, affecting cell behaviors such as proliferation and differentiation under various cellular environment parameters. An electrically conductive hydrogel pattern system mimics the native muscle tissue environment. In this study, we incorporated polyaniline (PANi) in a poly(ethylene glycol) (PEG) hydrogel matrix through UV-induced photolithography with photomasks, and electrically conductive hydrogel micropatterns were generated within a few seconds. The electrical conductance of the PANi/PEG hydrogel was 30.5 ± 0.5 mS/cm. C2C12 myoblasts were cultured on the resulting substrate, and the cells adhered selectively to the PANi/PEG hydrogel regions. Myogenic differentiation of the C2C12 cells was induced, and the alignment of myotubes was consistent with the arrangement of the line pattern. The expression of myosin heavy chain on the line pattern showed potential as a substrate for myogenic cell functionalization.
基于水凝胶基质的微图案可以通过图案形状和尺寸进行调整，在各种细胞环境参数下影响细胞行为，如增殖和分化。导电水凝胶图案系统模拟天然肌肉组织环境。在本研究中，我们通过使用光掩模的紫外诱导光刻技术将聚苯胺（PANi）掺入聚乙二醇（PEG）水凝胶基质中，并在几秒钟内生成了导电水凝胶微图案。PANi/PEG水凝胶的电导率为30.5±0.5 mS/cm。将C2C12成肌细胞培养在所得基质上，细胞选择性地粘附于PANi/PEG水凝胶区域。诱导了C2C12细胞的成肌分化，肌管的排列与线条图案的排列一致。线条图案上肌球蛋白重链的表达显示出作为成肌细胞功能化基质的潜力。
