Branco Cátia S, Scola Gustavo, Rodrigues Adriana D, Cesio Verónica, Heinzen Horacio, Godoy Alessandra, Funchal Cláudia, Coitinho Adriana S, Salvador Mirian
Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS 95070-560, Brazil.
Department of Natural Products and Pharmacognosy, Faculty of Chemistry, University of the Republic, Montevideo 11800, Uruguay.
Antioxidants (Basel). 2013 Jul 24;2(3):100-9. doi: 10.3390/antiox2030100.
Organic and conventional yerba mate (Ilex paraguariensis) is widely used in South America to prepare nonalcoholic drinks rich in polyphenols. These compounds are able to prevent the generation of reactive species, thus minimizing the incidence of several diseases. In this perspective, we hypothesized that yerba mate may have protective effects against pentylenetetrazol (PTZ)-induced oxidative damage in liver and serum of rats. Animals (n = 42) received distilled water (control) or yerba mate (organic or conventional) for fifteen days. Then, half of the rats of each group received 60 mg/kg PTZ intraperitoneally or saline solution. After 30 min the animals were euthanized and the liver and blood were collected. The results showed that organic and conventional yerba mate avoided PTZ-induced oxidative damage and nitric oxide production in the liver and serum of the rats. Moreover, both kinds of yerba mate prevented the decrease in enzymatic (superoxide dismutase and catalase) and non-enzymatic (sulfhydryl protein content) defenses in the liver and serum. In addition, histopathologic analysis of the liver showed that yerba mate reduced PTZ-induced cell damage. These findings indicate that yerba mate provides hepatoprotection and improves antioxidant status in the serum, which may contribute to the development of new therapeutic strategies using nutraceuticals drinks.
有机和传统的马黛茶（巴拉圭冬青）在南美洲被广泛用于制备富含多酚的非酒精饮料。这些化合物能够防止活性物质的产生，从而将多种疾病的发病率降至最低。从这个角度来看，我们假设马黛茶可能对戊四氮（PTZ）诱导的大鼠肝脏和血清氧化损伤具有保护作用。动物（n = 42）接受蒸馏水（对照组）或马黛茶（有机或传统）处理15天。然后，每组一半的大鼠腹腔注射60 mg/kg PTZ或生理盐水。30分钟后对动物实施安乐死并采集肝脏和血液。结果表明，有机和传统马黛茶可避免PTZ诱导的大鼠肝脏和血清氧化损伤及一氧化氮生成。此外，两种马黛茶均可防止肝脏和血清中酶（超氧化物歧化酶和过氧化氢酶）和非酶（巯基蛋白含量）防御能力的下降。此外，肝脏组织病理学分析表明，马黛茶可减轻PTZ诱导的细胞损伤。这些发现表明，马黛茶具有肝脏保护作用并可改善血清中的抗氧化状态，这可能有助于开发使用营养保健饮料的新治疗策略。
