Wang Xue, Wu Zhengjie, Wu Shengmei, Chen Xianxian, Hanif Misbah, Zhang Shengzhou
College of Life Sciences, Anhui Normal University, Wuhu, China.
College of Life Sciences, Anhui Normal University, Faisalabad, Pakistan.
PeerJ. 2021 Apr 26;9:e11234. doi: 10.7717/peerj.11234. eCollection 2021.
The argus snakehead ( Cantor) is a highly nutritious, freshwater, cultured bony fish with a high economic value. The health of the fish is closely related to its blood cells, which are critical for oxygen transport, natural defense, and immunity. We investigated the morphometry, microstructure, and cytochemical characteristics of the peripheral blood cells of . Our results may provide the basic reference values needed to monitor the health of this fish for large-scale cultivation.
The number of blood cells in were counted on a hemocytometer and their size was measured using a micrometer under light microscope. The morphology and classification of the blood cells were studied using Wright's staining and the cytochemical characteristics were studied using seven chemical stains including peroxidase (POX), Sudan black B (SBB), periodic acid-Schiff (PAS), acid phosphatase (ACP), alkaline phosphatase (ALP), chloroacetic acid AS-D naphthol esterase (AS-D), and -naphthol acetate esterase (-NAE).
The peripheral blood cells in can be classified as erythrocytes, leukocytes, and thrombocytes; of which, females had 2.9597 million/mm, 88,400/mm, and 43,600/mm, respectively, and males had 3.0105 million/mm, 105,500/mm, and 34,000/mm, respectively. Leukocytes consisted of neutrophils, monocytes, large lymphocytes, and small lymphocytes. Eosinophils and basophils were not found. Monocytes were the most numerous leukocytes identified, followed by neutrophils and small lymphocytes, while large lymphocytes were the least frequently identified. Cytochemical staining showed that erythrocytes were only positive for PAS staining. Neutrophils were strongly positive for POX, SBB, and ACP, and positive for all the other cytochemical stains. Monocytes were positive for PAS and -NAE and were weakly positive for ACP and AS-D staining. Large lymphocytes were positive for PAS and were weakly positive for ALP, AS-D, and -NAE staining. Small lymphocytes were positive for PAS and weakly positive for AS-D and -NAE staining. Thrombocytes were positive for PAS and were weakly positive for ACP and AS-D, but negative for the remaining cytochemical stains. The morphology of peripheral blood cells in was generally similar to that of other fish species, while the cytochemical staining patterns showed clear species specificity.
乌鳢（斑鳢）是一种营养丰富的淡水养殖硬骨鱼，具有很高的经济价值。鱼的健康状况与其血细胞密切相关，血细胞对氧气运输、天然防御和免疫至关重要。我们研究了乌鳢外周血细胞的形态计量学、微观结构和细胞化学特征。我们的结果可为大规模养殖中监测这种鱼的健康状况提供所需的基本参考值。
在血细胞计数板上计数乌鳢的血细胞数量，并在光学显微镜下使用测微计测量其大小。使用瑞氏染色研究血细胞的形态和分类，并使用包括过氧化物酶（POX）、苏丹黑B（SBB）、过碘酸希夫（PAS）、酸性磷酸酶（ACP）、碱性磷酸酶（ALP）、氯乙酸AS-D萘酚酯酶（AS-D）和α-萘酚乙酸酯酶（α-NAE）在内的七种化学染色剂研究细胞化学特征。
乌鳢外周血细胞可分为红细胞、白细胞和血小板；其中，雌性分别为295.97万/mm³、88400/mm³和43600/mm³，雄性分别为301.05万/mm³、105500/mm³和34000/mm³。白细胞由中性粒细胞、单核细胞、大淋巴细胞和小淋巴细胞组成。未发现嗜酸性粒细胞和嗜碱性粒细胞。单核细胞是鉴定出的数量最多的白细胞，其次是中性粒细胞和小淋巴细胞，而大淋巴细胞鉴定频率最低。细胞化学染色显示红细胞仅对PAS染色呈阳性。中性粒细胞对POX、SBB和ACP呈强阳性，对所有其他细胞化学染色呈阳性。单核细胞对PAS和α-NAE呈阳性，对ACP和AS-D染色呈弱阳性。大淋巴细胞对PAS呈阳性，对ALP、AS-D和α-NAE染色呈弱阳性。小淋巴细胞对PAS呈阳性，对AS-D和α-NAE染色呈弱阳性。血小板对PAS呈阳性，对ACP和AS-D呈弱阳性，但对其余细胞化学染色呈阴性。乌鳢外周血细胞的形态通常与其他鱼类相似，而细胞化学染色模式显示出明显的物种特异性。
