Gao Yingli, Qiang Lu, Wu Ni, Wang Hui, Hao Ying
Lab of Pathology and Immunology of Aquatic Animals, Jiangsu Ocean University, Lianyungang, 222005, China.
Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005, China.
Heliyon. 2024 Aug 27;10(17):e37011. doi: 10.1016/j.heliyon.2024.e37011. eCollection 2024 Sep 15.
The flounder () is a highly nutritious, cultured bony fish with a high economic value. The health of the fish is closely related to its blood cells, which are critical for oxygen transport, natural defense, and immunity. The microstructures, classification, counting and size of peripheral blood cells in . were observed and measured by Wright-Giemsa staining, and the cytochemical characteristics of peripheral blood cells were investigated by different cytochemical staining methods including periodic acid-Schiff (PAS), Sudan black B (SBB), acid phosphatase (ACP), alkaline phosphatase (ALP), peroxidase (POX), and α-naphthol acetate esterase (NAE). Besides, the ultrastructures of different cells were detected by the transmission electron microscope. The results showed that erythrocytes, thrombocytes, lymphocytes, monocytes, and neutrophils constituted the peripheral blood cells in . . More heterochromatins were found in erythrocytes, thrombocytes and neutrophils; however, more organelles with fewer heterochromatins were found in monocytes. Endoplasmic reticulums and phagocytic vesicles were abundant in neutrophils. Lymphocytes were the most abundant in leukocytes, followed by monocytes and neutrophils. The cytochemical staining results showed that all leukocytes were positive for SBB. Most of the lymphocytes were positive for PAS, and monocytes were positive for PAS, ACP, and POX. As for neutrophils, ACP and POX were positive. Both monocytes and neutrophils showed positive for SBB, ACP and POX, indicating that the two kinds of cells play a vital role in phagocytosis and bactericidal action. Only lymphocytes were positive for ALP, indicating that they were important in inflammation and immune response. Some characteristics similarities in peripheral blood cells were showed in . just as the other fishes.
比目鱼是一种营养丰富、可养殖的具有高经济价值的硬骨鱼。鱼的健康与其血细胞密切相关，血细胞对于氧气运输、天然防御和免疫至关重要。通过瑞氏 - 吉姆萨染色观察和测量了比目鱼外周血细胞的微观结构、分类、计数和大小，并通过不同的细胞化学染色方法，包括过碘酸 - 希夫反应（PAS）、苏丹黑B（SBB）、酸性磷酸酶（ACP）、碱性磷酸酶（ALP）、过氧化物酶（POX）和α - 萘酚醋酸酯酶（NAE），研究了外周血细胞的细胞化学特征。此外，通过透射电子显微镜检测了不同细胞的超微结构。结果表明，红细胞、血小板、淋巴细胞、单核细胞和嗜中性粒细胞构成了比目鱼的外周血细胞。在红细胞、血小板和嗜中性粒细胞中发现更多异染色质；然而，在单核细胞中发现更多细胞器且异染色质较少。嗜中性粒细胞中内质网和吞噬泡丰富。淋巴细胞在白细胞中最为丰富，其次是单核细胞和嗜中性粒细胞。细胞化学染色结果表明，所有白细胞的SBB染色均呈阳性。大多数淋巴细胞的PAS染色呈阳性，单核细胞的PAS、ACP和POX染色呈阳性。至于嗜中性粒细胞，ACP和POX染色呈阳性。单核细胞和嗜中性粒细胞的SBB、ACP和POX染色均呈阳性，表明这两种细胞在吞噬作用和杀菌作用中起重要作用。只有淋巴细胞的ALP染色呈阳性，表明它们在炎症和免疫反应中很重要。与其他鱼类一样，比目鱼外周血细胞也表现出一些特征相似性。
