Afzali Daryoush, Fathirad Fariba, Ghaseminezhad Sima
Department of Environment, Institute of Science and High Technology and Environmental Sciences, Graduate University of advanced Technology, Kerman, Iran.
Chemistry Department, Shahid Bahonar University of Kerman, Kerman, Iran.
J Food Sci Technol. 2016 Jan;53(1):909-14. doi: 10.1007/s13197-015-2016-8. Epub 2015 Sep 16.
In the present study, a carbon paste electrode chemically modified with gold nanoparticles was used as a sensitive electrochemical sensor for determination of ochratoxin A. The differential pulse voltammetric method was employed to study the behavior of ochratoxin A on this modified electrode. The effect of variables such as percent of gold nanoparticles, pH of sample solution, accumulation potential and time on voltammogram peak current were optimized. The proposed electrode showed good oxidation response for ochratoxin A in 0.1 mol L(-1) PBS (pH 7.2) and the peak potential was about +0.8 V (vs. Ag/AgCl). The peak current increased linearly with the ochratoxin A concentration in the range of 0.5-100 nM. The detection limit was found to be 0.2 nM and the relative standard deviation was 6.2 % (n = 7). The method has been applied to the determination of ochratoxin A in cereal derived products such as breakfast cereals, cereal-based baby foods and beer samples.
在本研究中，用金纳米粒子化学修饰的碳糊电极被用作测定赭曲霉毒素A的灵敏电化学传感器。采用差分脉冲伏安法研究赭曲霉毒素A在该修饰电极上的行为。优化了诸如金纳米粒子的百分比、样品溶液的pH值、富集电位和时间等变量对伏安图峰电流的影响。所提出的电极在0.1 mol L(-1) PBS（pH 7.2）中对赭曲霉毒素A表现出良好的氧化响应，峰电位约为+0.8 V（相对于Ag/AgCl）。峰电流在0.5 - 100 nM范围内随赭曲霉毒素A浓度呈线性增加。检测限为0.2 nM，相对标准偏差为6.2%（n = 7）。该方法已应用于谷物衍生产品如早餐谷物、谷物基婴儿食品和啤酒样品中赭曲霉毒素A的测定。
