The Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Ak. Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
The Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, Slovakia.
Toxins (Basel). 2020 Nov 24;12(12):739. doi: 10.3390/toxins12120739.
According to the EU legislation, ochratoxin A contamination is controlled in wines. Tokaj wine is a special type of sweet wine produced from botrytized grapes infected by "noble rot" . Although a high contamination was reported in sweet wines and noble rot grapes could be susceptible to coinfection with other fungi, including ochratoxigenic species, no screening of Tokaj wines for mycotoxin contamination has been carried out so far. Therefore, we developed an analytical method for the determination of ochratoxin A (OTA) and ochratoxin B (OTB) involving online SPE coupled to HPLC-FD using column switching to achieve the fast and sensitive control of mycotoxin contamination. The method was validated with recoveries ranging from 91.6% to 99.1% with an RSD less than 2%. The limits of quantification were 0.1 and 0.2 µg L for OTA and OTB, respectively. The total analysis time of the online SPE-HPLC-FD method was a mere 6 min. This high throughput enables routine analysis. Finally, we carried out an extensive investigation of the ochratoxin contamination in 59 Slovak Tokaj wines of 1959-2017 vintage. Only a few positives were detected. The OTA content in most of the checked wines did not exceed the EU maximum tolerable limit of 2 µg L, indicating a good quality of winegrowing and storing.
根据欧盟法规，对葡萄酒中的赭曲霉毒素 A 污染进行了控制。托卡伊葡萄酒是一种特殊的甜酒，由感染“贵族腐烂”的贵腐葡萄制成。尽管甜酒中报告了高污染，并且贵腐葡萄可能容易受到其他真菌（包括产赭曲霉毒素的物种）的合并感染，但迄今为止尚未对托卡伊葡萄酒进行霉菌毒素污染的筛选。因此，我们开发了一种用于测定赭曲霉毒素 A（OTA）和赭曲霉毒素 B（OTB）的分析方法，涉及在线 SPE 与 HPLC-FD 结合使用柱切换，以实现对霉菌毒素污染的快速和灵敏控制。该方法的回收率范围为 91.6%至 99.1%，RSD 小于 2%。定量限分别为 OTA 和 OTB 的 0.1 和 0.2 µg L。在线 SPE-HPLC-FD 方法的总分析时间仅为 6 分钟。这种高通量能够进行常规分析。最后，我们对 1959 年至 2017 年的 59 种斯洛伐克托卡伊葡萄酒进行了广泛的赭曲霉毒素污染调查。仅检测到少数阳性。大多数检查葡萄酒中的 OTA 含量未超过欧盟 2 µg L 的最大允许限量，表明酿酒和储存质量良好。
