尿苷对骨骼肌细胞中UTP和糖原合成的影响：胰岛素对UTP形成的刺激作用。
Effect of uridine on cellular UTP and glycogen synthesis in skeletal muscle: stimulation of UTP formation by insulin.
作者信息
Haugaard E S, Frantz K B, Haugaard N
出版信息
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2339-42. doi: 10.1073/pnas.74.6.2339.
Abstract
The relation between cellular uracil nucleotides and ability to synthesize glycogen was studied in rat diaphragm incubated in vitro. In the absence of exogenous uridine the tissue content of UTP and rate of glycogen synthesis decreased with time. Uridine added to the medium increased cellular UTP and UDPG and stimulated glycogen synthesis. Insulin significantly increased the synthesis of UTP from extracellular uridine. This action of insulin appeared to be due to a stimulation of phosphorylation of the nucleoside and not to an effect on transport at the concentrations of uridine studied. However, an effect of insulin on transport of uridine at low concentrations cannot be excluded.
摘要
在体外培养的大鼠膈肌中研究了细胞尿嘧啶核苷酸与糖原合成能力之间的关系。在没有外源性尿苷的情况下，UTP的组织含量和糖原合成速率随时间下降。添加到培养基中的尿苷增加了细胞内的UTP和UDPG，并刺激了糖原合成。胰岛素显著增加了从细胞外尿苷合成UTP的量。胰岛素的这一作用似乎是由于刺激了核苷的磷酸化，而不是在所研究的尿苷浓度下对转运的影响。然而，不能排除胰岛素在低浓度下对尿苷转运的影响。
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