Gilbert-Kawai Edward, Coppel Jonny, Bountziouka Vassiliki, Ince Can, Martin Daniel
University College London Centre for Altitude Space and Extreme Environment Medicine, UCLH NIHR Biomedical Research Centre, Institute of Sport and Exercise Health, 170 Tottenham Court Road, London, W1T 7HA, UK.
Statistical Support Service, Population, Policy and Practice Programme, Institute of Child Health, University College London, London, England.
BMC Med Imaging. 2016 Jan 21;16:10. doi: 10.1186/s12880-015-0078-8.
The 'Cytocam' is a third generation video-microscope, which enables real time visualisation of the in vivo microcirculation. Based upon the principle of incident dark field (IDF) illumination, this hand held computer-controlled device was designed to address the technical limitations of its predecessors, orthogonal polarization spectroscopy and sidestream dark field (SDF) imaging. In this manuscript, we aimed to compare the quality of sublingual microcirculatory image acquisition between the IDF and SDF devices.
Using the microcirculatory image quality scoring (MIQS) system, (six categories scored as either 0 = optimal, 1 = acceptable, or 10 = unacceptable), two independent raters compared 30 films acquired using the Cytocam IDF video-microscope, to an equal number obtained with an SDF device. Blinded to the origin of the films, the raters were therefore able to score between 0 and 60 for each film analysed. The scores' distributions between the two techniques were compared.
The median MIQS (95% CI) given to the SDF camera was 7 (1.5-12), as compared to 1 (0.5-1.0) for the IDF device (p < 0.0001). Of the six categories assessed by the MIQS, nearly one fifth of the SDF videos were scored as unacceptable for pressure (20%), content (20%), and stability (17%), with focus scoring deficiently 13% of the time. High agreement between the two raters scoring values was evident, with an intra-class correlation coefficient (ICC) of 0.96 (95% CI: 0.94, 0.98).
These results demonstrate that the quality of sublingual microcirculatory image acquisition is superior in the Cytocam IDF video-microscope, as compared to the SDF video-microscope.
“细胞相机”是第三代视频显微镜，能够实时可视化体内微循环。基于入射暗场（IDF）照明原理，这款手持式计算机控制设备旨在解决其前身——正交偏振光谱和侧流暗场（SDF）成像技术的局限性。在本论文中，我们旨在比较IDF设备和SDF设备舌下微循环图像采集的质量。
使用微循环图像质量评分（MIQS）系统（六个类别，评分分别为0 = 最佳、1 = 可接受或10 = 不可接受），两名独立评估者将使用细胞相机IDF视频显微镜获取的30段影像，与使用SDF设备获取的数量相等的影像进行比较。评估者对影像来源不知情，因此能够对每段分析的影像在0至60分之间进行评分。比较了两种技术评分的分布情况。
SDF相机的MIQS中位数（95%置信区间）为7（1.5 - 12），而IDF设备为1（0.5 - 1.0）（p < 0.0001）。在MIQS评估的六个类别中，近五分之一的SDF视频在压力（20%）、内容（20%）和稳定性（17%）方面被评为不可接受，聚焦评分不足的情况占13%。两名评估者的评分值之间具有高度一致性，组内相关系数（ICC）为0.96（95%置信区间：0.94，0.98）。
这些结果表明，与SDF视频显微镜相比，细胞相机IDF视频显微镜舌下微循环图像采集的质量更优。
