Moradi-Kor Nasroallah, Ghanbari Ali, Rashidipour Hadi, Bandegi Ahmad Reza, Yousefi Behpour, Barati Mehdi, Kokhaei Parviz, Rashidy-Pour Ali
Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran.
Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran.
J Exp Pharmacol. 2020 Mar 19;12:75-85. doi: 10.2147/JEP.S237378. eCollection 2020.
The amygdala structural and functional abnormalities have been implicated in numerous neuropsychiatric and neurodevelopmental disorders. Given the important role of the amygdala in stress responses and the susceptibility of the females to adolescent stress, the present study investigated the beneficial effects of microalgae (SP) as a neuroprotective supplement against adolescent stress-induced oxidative stress, brain-derived neurotrophic factor (BDNF) alterations, molecular and morphological remodeling in the basolateral amygdala (BLA) of adult female rats.
During the adolescent period (PNDs 30-40) rats were subjected to restraint stress (2 h/day for 10 days). Then, the animals were subjected to 15 days treatment (PNDs 41-55) with SP (200 mg/kg/day) followed by biochemical (BDNF and stress oxidative markers), molecular (BDNF and its receptor tropomyosin receptor kinase B [TrkB] mRNA expression), and morphological (dendritic length and spines) assessments in the BLA.
The study revealed that adolescent stress decreased BDNF levels and reduced apical dendritic length and branch points of pyramidal neurons in the BLA. In addition, chronic stress significantly increased oxidative stress parameters and decreased BDNF and TrkB mRNA expression in the BLA. Treatment with SP alleviated both biochemical, molecular, and neuroanatomical deficits that induced by adolescent stress.
Our findings provide important evidence that SP as a non-pharmacological intervention during adolescent period can protect against chronic stress-induced neuroanatomical biochemical, and molecular deficits in adulthood, and thus, reduce stress-related disorders.
杏仁核的结构和功能异常与多种神经精神疾病和神经发育障碍有关。鉴于杏仁核在应激反应中的重要作用以及雌性对青春期应激的易感性，本研究调查了微藻（SP）作为一种神经保护补充剂，对成年雌性大鼠青春期应激诱导的氧化应激、脑源性神经营养因子（BDNF）改变、基底外侧杏仁核（BLA）的分子和形态重塑的有益作用。
在青春期（出生后第 30 - 40 天），大鼠接受束缚应激（每天 2 小时，共 10 天）。然后，动物接受 15 天的 SP 治疗（出生后第 41 - 55 天，200 mg/kg/天），随后对 BLA 进行生化（BDNF 和应激氧化标志物）、分子（BDNF 及其受体原肌球蛋白受体激酶 B [TrkB] mRNA 表达）和形态学（树突长度和棘）评估。
研究表明，青春期应激降低了 BLA 中 BDNF 的水平，减少了锥体神经元顶端树突的长度和分支点。此外，慢性应激显著增加了氧化应激参数，并降低了 BLA 中 BDNF 和 TrkB mRNA 的表达。SP 治疗减轻了青春期应激诱导的生化、分子和神经解剖学缺陷。
我们的研究结果提供了重要证据，表明 SP 作为青春期的一种非药物干预措施，可以预防成年期慢性应激诱导的神经解剖学、生化和分子缺陷，从而减少与应激相关的疾病。
