Sathishkumar Palanivel, Vennila Krishnan, Jayakumar Rajarajeswaran, Yusoff Abdull Rahim Mohd, Hadibarata Tony, Palvannan Thayumanavan
Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor, Malaysia.
Laboratory of Bioprocess and Engineering, Department of Biochemistry, Periyar University, Salem, 636 011, Tamil Nadu, India.
Bioprocess Biosyst Eng. 2016 Apr;39(4):651-9. doi: 10.1007/s00449-016-1546-4. Epub 2016 Jan 22.
In this study, phyto-synthesis of silver nanoparticles (AgNPs) was achieved using an aqueous leaf extract of Alternanthera tenella. The phytochemical screening results revealed that flavonoids are responsible for the AgNPs formation. The AgNPs were characterised using UV-visible spectrophotometer, field emission scanning microscopy/energy dispersive X-ray, transmission electron microscopy, fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction. The average size of the nanoparticles was found to be ≈48 nm. The EDX results show that strong signals were observed for the silver atoms. The strong band appearing at 1601-1595 cm(-1) correspond to C-C stretching vibration from dienes in FT-IR spectrum indicating the formation of AgNPs. Human breast adenocarcinoma (MCF-7) cells treated with various concentrations of AgNPs showed a dose-dependent increase in cell inhibition. The IC50 value of the AgNPs was calculated to be 42.5 μg mL(-1). The AgNPs showed a significant reduction in the migration of MCF-7 cells.
在本研究中，使用细叶虾钳菜的水提叶提取物实现了银纳米颗粒（AgNPs）的植物合成。植物化学筛选结果表明，黄酮类化合物是AgNPs形成的原因。使用紫外可见分光光度计、场发射扫描显微镜/能量色散X射线、透射电子显微镜、傅里叶变换红外光谱（FT-IR）和X射线衍射对AgNPs进行了表征。发现纳米颗粒的平均尺寸约为48nm。EDX结果表明，观察到银原子的强信号。在FT-IR光谱中，出现在1601 - 1595 cm(-1)处的强带对应于二烯中C-C的伸缩振动，表明AgNPs的形成。用不同浓度的AgNPs处理人乳腺腺癌（MCF-7）细胞后，细胞抑制呈剂量依赖性增加。计算出AgNPs的IC50值为42.5μg mL(-1)。AgNPs显著降低了MCF-7细胞的迁移。
