Shershakova Nadezda, Baraboshkina Elena, Andreev Sergey, Purgina Daria, Struchkova Irina, Kamyshnikov Oleg, Nikonova Alexandra, Khaitov Musa
NRC Institute of Immunology FMBA of Russia, Moscow, Russia.
J Nanobiotechnology. 2016 Jan 25;14:8. doi: 10.1186/s12951-016-0159-z.
Water-soluble form of fullerene C60 is a promising tool for the control of ROS-dependent inflammation including allergic diseases. Anti-inflammatory effects of C60 (nC60) aqueous dispersion were evaluated in the mouse models of atopic dermatitis using subcutaneous (SC) and epicutaneous (EC) applications during 50 days period. A highly stable nC60 was prepared by exhaustive dialysis of water-organic C60 solution against water, where the size and ζ-potential of fullerene nanoparticles are about 100 nm and -30 mV, respectively.
To induce skin inflammation, female BALB/c mice were EC sensitized with ovalbumin three times during one-weekly exposures. The nC60 solution was administrated in mice subcutaneously (SC) (0.1 mg/kg) and epicutaneously (EC) (1 mg/kg). Significant suppression of IgE and Th2 cytokines production and a concomitant rise in concentrations of Th1 cytokines were observed in nC60-treated groups. In addition, a significant increase in the levels of Foxp3(+) and filaggrin mRNA expression was observed at EC application. Histological examination of skin samples indicated that therapeutic effect was achieved by both EC and SC treatment, but it was more effective with EC. Pronounced reduction of the eosinophil and leukocyte infiltration in treated skin samples was observed.
We suppose that nC60 treatment shifts immune response from Th2 to Th1 and restores to some extent the function of the skin barrier. This approach can be a good alternative to the treatment of allergic and other inflammatory diseases.
富勒烯C60的水溶性形式是控制包括过敏性疾病在内的活性氧依赖性炎症的一种有前景的工具。在50天的时间里，通过皮下（SC）和表皮（EC）应用，在特应性皮炎小鼠模型中评估了C60（nC60）水分散体的抗炎作用。通过将水-有机C60溶液对水进行彻底透析制备了高度稳定的nC60，其中富勒烯纳米颗粒的尺寸和ζ电位分别约为100nm和-30mV。
为了诱导皮肤炎症，雌性BALB/c小鼠在每周一次的暴露期间用卵清蛋白进行三次表皮致敏。将nC60溶液皮下（SC）（0.1mg/kg）和表皮（EC）（1mg/kg）给予小鼠。在nC60处理组中观察到IgE和Th2细胞因子产生的显著抑制以及Th1细胞因子浓度的相应升高。此外，在表皮应用时观察到Foxp3(+)和丝聚蛋白mRNA表达水平显著增加。皮肤样本的组织学检查表明，表皮和皮下治疗均取得了治疗效果，但表皮治疗更有效。在处理过的皮肤样本中观察到嗜酸性粒细胞和白细胞浸润明显减少。
我们推测nC60治疗可使免疫反应从Th2转变为Th1，并在一定程度上恢复皮肤屏障功能。这种方法可能是治疗过敏性和其他炎症性疾病的一种良好替代方案。
