荧光标记的吗啉代核苷三磷酸衍生物的有效合成
Effective Synthesis of Fluorescently Labeled Morpholino Nucleoside Triphosphate Derivatives.
作者信息
Tarasenko Yuliya V, Abramova Tatyana V, Mamatuk Viktor I, Silnikov Vladimir N
机构信息
a Institute of Chemical Biology and Fundamental Medicine SB RAS , Lavrent'ev Ave 8, Novosibirsk 630090 , Russian Federation.
b Institute of Organic Chemistry SB RAS , Lavrent'ev Ave 9, Novosibirsk 630090 , Russian Federation.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2016;35(1):32-42. doi: 10.1080/15257770.2015.1114125. Epub 2016 Jan 25.
Morpholino nucleoside triphosphates (A, U, G, C, T) bearing the active functional amino group tethered to morpholine residue and their fluorescently labeled derivatives were synthesized. All compounds were characterized by (1)H, (13)C, and (31)P NMR, and mass spectrometry. A possibility of using fluorescently labeled morpholino nucleoside triphosphates as chain terminators in DNA sequencing is discussed.
合成了带有连接到吗啉环残基上的活性官能氨基的吗啉代核苷三磷酸（A、U、G、C、T）及其荧光标记衍生物。所有化合物均通过¹H、¹³C和³¹P核磁共振以及质谱进行了表征。讨论了在DNA测序中使用荧光标记的吗啉代核苷三磷酸作为链终止剂的可能性。
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