Vara J, Lewandowska-Skarbek M, Wang Y G, Donadio S, Hutchinson C R
School of Pharmacy, University of Wisconsin, Madison 53706.
J Bacteriol. 1989 Nov;171(11):5872-81. doi: 10.1128/jb.171.11.5872-5881.1989.
Genes that govern the formation of deoxysugars or their attachment to erythronolide B and 3 alpha-mycarosyl erythronolide B, intermediates of the biosynthesis of the 14-membered macrolide antibiotic erythromycin, were cloned from Saccharopolyspora erythraea (formerly Streptomyces erythreus). Segments of DNA that complement the eryB25, eryB26, eryB46, eryC1-60, and eryD24 mutations blocking the formation of erythronolide B or 3 alpha-mycarosyl erythronolide B, when cloned in Escherichia coli-Streptomyces shuttle cosmids or plasmid vectors that can transform S. erythraea, were located in a ca. 18-kilobase-pair region upstream of the erythromycin resistance (ermE) gene. The eryC1 gene lies just to the 5' side of ermE, and one (or possibly two) eryB gene is approximately 12 kilobase pairs farther upstream. Another eryB gene may be in the same region, while an additional eryB mutation appears to be located elsewhere. The eryD gene lies between the eryB and eryC1 genes and may regulate their function on the basis of the phenotype of an EryD- mutant.
调控脱氧糖形成或其与红霉内酯B及3-α- mycarosyl红霉内酯B（14元大环内酯类抗生素红霉素生物合成的中间体）连接的基因，是从糖多孢红霉菌（以前称为红链霉菌）中克隆得到的。当克隆于可转化糖多孢红霉菌的大肠杆菌-链霉菌穿梭黏粒或质粒载体中时，能互补阻断红霉内酯B或3-α- mycarosyl红霉内酯B形成的eryB25、eryB26、eryB46、eryC1-60和eryD24突变的DNA片段，位于红霉素抗性（ermE）基因上游约18千碱基对的区域。eryC1基因位于ermE的5'端，一个（或可能两个）eryB基因位于其上游约12千碱基对处。另一个eryB基因可能在同一区域，而另一个eryB突变似乎位于其他地方。eryD基因位于eryB和eryC1基因之间，基于EryD-突变体的表型，它可能调控它们的功能。
