[云南昆明静脉吸毒人群丙型肝炎病毒基因分型分析]

[Analysis on genotypes of hepatitis C virus among intravenous drug users in Kunming, Yunnan].

作者信息

Chen Min, Ma Yanling, Luo Hongbing, Chen Huichao, Lu Ran, Dai Jie, Song Lijun, Yang Chaojun, Mei Jingyuan, Jia Manhong

机构信息

Institute of AIDS/STD Control and Prevention, Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.

Institute of AIDS/STD Control and Prevention, Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China; Email:

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2015 Sep;36(9):994-7.

PMID:26814869

Abstract

OBJECTIVE

To investigate the distribution of hepatitis C virus (HCV) subtypes among intravenous drug users (IDUs) in Kunming, Yunnan province, in 2014.

METHODS

A total of 276 plasma samples were collected from IDUs in Kunming during April 2014 -July 2014, in which 199 plasma samples were tested to be HCV antibody positive. For the HCV antibody positive samples, HCV E1E2 genes and NS5B genes were amplified by using nested-polymerase chain reaction (PCR). After sequencing, the HCV subtypes were determined through phylogenetic analysis.

RESULTS

Based on the phylogenetic trees of E1E2 gene and NS5B gene fragments, a total of 125 samples were genotyped. 3b was the predominant subtype (48.8%,61/125), followed by 3a (30.4%, 38/125), 6n (14.4%, 18/125), 6a (3.2%, 4/125) and 1b (3.2%, 4/125). The distributions of HCV subtypes by sex, marital status, ethnic group and HIV-1 infection status showed no statistical differences. However, the distribution of HCV subtypes by age showed statistical difference, the diversity of HCV subtypes was found in age group <45 years. The genetic distances of 3a, 3b and 6a on E1E2 and NS5B were larger than those of 1b and 6n. For genotype 3, the genetic distances of 3b on E1E2 and NS5B were larger than those of 3a.

CONCLUSION

Five HCV subtypes were found among IDUs in Kunming, HCV subtypes 3b and 3a were predominant, which have circulated in this population for long time.

摘要

目的

调查2014年云南省昆明市静脉吸毒者中丙型肝炎病毒（HCV）亚型的分布情况。

方法

2014年4月至7月期间，从昆明市的静脉吸毒者中总共采集了276份血浆样本，其中199份血浆样本检测出HCV抗体呈阳性。对于HCV抗体呈阳性的样本，采用巢式聚合酶链反应（PCR）扩增HCV E1E2基因和NS5B基因。测序后，通过系统发育分析确定HCV亚型。

结果

根据E1E2基因和NS5B基因片段的系统发育树，共对125个样本进行了基因分型。3b是主要亚型（48.8%，61/125），其次是3a（30.4%，38/125）、6n（14.4%，18/125）、6a（3.2%，4/125）和1b（3.2%，4/125）。HCV亚型按性别、婚姻状况、民族和HIV-1感染状况的分布无统计学差异。然而，HCV亚型按年龄的分布存在统计学差异，在<45岁年龄组中发现了HCV亚型的多样性。3a、3b和6a在E1E2和NS5B上的遗传距离大于1b和6n。对于基因3型，3b在E1E2和NS5B上的遗传距离大于3a。