Silva Rosilda Mendes da, Malafaia Osvaldo, Torres Orlando Jorge Martins, Czeczko Nicolau Gregori, Marinho Junior Carlos Hespanha, Kozlowski Ronaldo Kiviatcoski
Hospital Universitário Evangélico de Curitiba, Curitiba, PR, Brasil.
Hospital São Domingos, São Luis, MA, Brasil.
Rev Col Bras Cir. 2015 Nov-Dec;42(6):393-7. doi: 10.1590/0100-69912015006008.
to evaluate liver regeneration in rats after partial hepatectomy of 60% with and without action diet supplemented with fatty acids through the study of the regenerated liver weight, laboratory parameters of liver function and histological study.
thirty-six Wistar rats, males, adults were used, weighing between 195 and 330 g assigned to control and groups. The supplementation group received the diet by gavage and were killed after 24h, 72h and seven days. Evaluation of regeneration occurred through analysis of weight gain liver, serum aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidase, and mitosis of the liver stained with H&E.
the diet supplemented group showed no statistical difference (p>0.05) on the evolution of weights. Administration of fatty acids post-hepatectomy had significant reduction in gamma glutamyltransferase levels and may reflect liver regeneration. Referring to mitotic index, it did not differ between period of times among the groups.
supplementation with fatty acids in rats undergoing 60% hepatic resection showed no significant interference related to liver regeneration.
通过研究再生肝脏重量、肝功能实验室参数及组织学，评估60%部分肝切除术后补充脂肪酸的作用饮食和未补充脂肪酸的大鼠的肝脏再生情况。
使用36只体重在195至330克之间的成年雄性Wistar大鼠，分为对照组和实验组。补充组通过灌胃给予饮食，并在24小时、72小时和7天后处死。通过分析肝脏重量增加、血清天冬氨酸转氨酶、丙氨酸转氨酶、γ-谷氨酰转肽酶以及用苏木精和伊红染色的肝脏有丝分裂来评估再生情况。
补充饮食组在体重变化方面无统计学差异（p>0.05）。肝切除术后给予脂肪酸可使γ-谷氨酰转移酶水平显著降低，这可能反映肝脏再生情况。就有丝分裂指数而言，各组不同时间之间无差异。
在接受60%肝切除术的大鼠中补充脂肪酸对肝脏再生无显著干扰。
