Firat Ozgur, Makay Ozer, Yeniay Levent, Gokce Goksel, Yenisey Cigdem, Coker Ahmet
Department of General Surgery, Ege University Hospital, Izmir, Turkey.
Department of Pharmacology, Faculty of Pharmacy, Ege University, Izmir, Turkey.
Ann Surg Treat Res. 2017 Jul;93(1):1-10. doi: 10.4174/astr.2017.93.1.1. Epub 2017 Jun 26.
Lipid peroxidation and consequent reactive oxygen species in the setting of oxidative stress have crucial roles in liver regeneration, which may adversely affect the regeneration itself and lead to liver failure. The aim of the current study is to investigate whether omega-3 fatty acid supplementation inhibits oxidative stress in an experimental model of liver regeneration.
Forty rats were allocated to four groups. Rats in group A received a sham operation. Rats in group B were subjected to right portal vein ligation (RPVL) and saline infusion. Rats in groups C and D were subjected to RPVL and total parenteral nutrition (TPN) with an all-in-one admixture containing a soybean oil based lipid emulsion. Rats in group D were additionally supplemented with omega-3 fatty acid infusion. Oxidative stresses in the blood and liver were measured by glutathione, superoxide dismutase, catalase, glutathione peroxidase, malondialdehyde, and nitric oxide.
Omega-3 supplementation to the TPN solution significantly corrected alterations in the blood and tissue concentrations of oxidants and anti-oxidants during regeneration (P < 0.05).
Omega-3 fatty acid supplementation to the TPN solution revealed promising results in removal of oxidative stress that emerges during liver regeneration.
在氧化应激环境下，脂质过氧化及随之产生的活性氧在肝再生中起关键作用，这可能对再生本身产生不利影响并导致肝衰竭。本研究的目的是探讨在肝再生实验模型中补充ω-3脂肪酸是否能抑制氧化应激。
将40只大鼠分为四组。A组大鼠接受假手术。B组大鼠接受右门静脉结扎（RPVL）并输注生理盐水。C组和D组大鼠接受RPVL并通过含大豆油基脂质乳剂的全合一混合液进行全胃肠外营养（TPN）。D组大鼠额外补充ω-3脂肪酸输注。通过谷胱甘肽、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、丙二醛和一氧化氮来测量血液和肝脏中的氧化应激。
在TPN溶液中补充ω-3能显著纠正再生过程中血液和组织中氧化剂和抗氧化剂浓度的变化（P<0.05）。
在TPN溶液中补充ω-3脂肪酸在消除肝再生过程中出现的氧化应激方面显示出有前景的结果。
