Moazed D, Noller H F
Thimann Laboratory, University of California, Santa Cruz 95064.
Nature. 1989 Nov 9;342(6246):142-8. doi: 10.1038/342142a0.
Direct chemical 'footprinting' shows that translocation of transfer RNA occurs in two discrete steps. During the first step, which occurs spontaneously after the formation of the peptide bond, the acceptor end of tRNA moves relative to the large ribosomal subunit resulting in 'hybrid states' of binding. During the second step, which is promoted by elongation factor EF-G, the anticodon end of tRNA, along with the messenger RNA, moves relative to the small ribosomal subunit.
直接化学“足迹法”表明，转运RNA的易位发生在两个离散步骤中。在第一步中，肽键形成后自发发生，tRNA的受体端相对于大核糖体亚基移动，导致结合的“杂交状态”。在第二步中，由延伸因子EF-G促进，tRNA的反密码子端与信使RNA一起相对于小核糖体亚基移动。
