Zhao Guodong, Xu Liang, Hui Limei, Zhao Jianjun
Department of General Surgery, Affiliated Hospital of Hebei University of Engineering Handan 056002, Hebei, China.
Department of Obstetrics, Affiliated Hospital of Hebei University of Engineering Handan 056002, Hebei, China.
Int J Clin Exp Pathol. 2015 Nov 1;8(11):14252-6. eCollection 2015.
As one of the most popular and deadly malignant tumors, gastric cancer still has difficulty in early-diagnosis. Recently the level of circulated DNA related with tumors can be used for diagnosis. MicroRNA-421 (miR-421) has been found to be up-regulated in tumor cells. Whether peripheral miR-421 can be used as a marker for diagnosis of gastric carcinoma, however, remains unclear. The expression level of miR-421 in both gastric cancer and normal people were firstly quantified. We then performed in vitro transfection of gastric carcinoma cell line to potentiate or silence miR-421 level. Cell apoptosis and apoptotic protein levels were quantified by flow cytometry and Western blotting, respectively. MiR-421 level in the peripheral blood of gastric cancer patients was significantly elevated. In gastric cancer cell line, the up-regulation of miR-421 significantly inhibited cell apoptosis. The silencing of miR-421 promoted cell apoptosis. Such anti-apoptotic role of miR-421 was accomplished by inhibiting caspase 3, up-regulating Bcl-2 and inhibiting Bax. MiR-421 was up-regulated in both tumor tissue and peripheral blood, and can modulate cell apoptosis. Circulated miR-421 can work as a serological marker for early diagnosis of gastric cancer.
作为最常见且致命的恶性肿瘤之一，胃癌在早期诊断方面仍存在困难。近年来，与肿瘤相关的循环DNA水平可用于诊断。已发现MicroRNA-421（miR-421）在肿瘤细胞中上调。然而，外周血中的miR-421是否可作为胃癌诊断的标志物仍不清楚。首先对胃癌患者和正常人中miR-421的表达水平进行了定量分析。然后对胃癌细胞系进行体外转染，以增强或沉默miR-421水平。分别通过流式细胞术和蛋白质免疫印迹法对细胞凋亡和凋亡蛋白水平进行定量分析。胃癌患者外周血中的miR-421水平显著升高。在胃癌细胞系中，miR-421的上调显著抑制细胞凋亡。miR-421的沉默促进细胞凋亡。miR-421的这种抗凋亡作用是通过抑制半胱天冬酶3、上调Bcl-2和抑制Bax来实现的。miR-421在肿瘤组织和外周血中均上调，且可调节细胞凋亡。循环miR-421可作为胃癌早期诊断的血清学标志物。
