Popov T M, Dikov T, Goranova T E, Stancheva G, Kaneva R P, Todorov S, Stoyanov O, Rangachev J, Marinov T, Popova D P, Mitev V I, Konov D
Department of ENT, Medical University of Sofia, Bulgaria;
Department of Pathology, Medical University of Sofia, Bulgaria.
Acta Otorhinolaryngol Ital. 2015 Oct;35(5):321-4. doi: 10.14639/0392-100X-588.
Angiogenesis is one of the six originally constituted hallmarks of cancer that has been extensively studied in the last two decades. The aim of our study is to assess the microvessel and macrophageal density in laryngeal carcinoma and its clinicopathological correlations. We immunohistochemically assessed microvessel density (CD34) and macrophage count (CD68) using microarray techniques and then looked for clinicopathological correlations. The mean micro-vessel density in the study group was 14.27 ± 12.92 vessels in a ×200 field with a mean macrophageal infiltration density of 5.19 ± 4.32. Median microvessel density was significantly higher in patients with metastasis than in patients without metastasis. Additionally, linear regression established that macrophageal infiltration density could predict microvessel density in laryngeal carcinoma. We found no association between either factor and recurrence rate or other clinical characteristics. Our study adds additional data to a problem that has been widely studied during the last two decades, even if controversies in this area still remain.
血管生成是最初构成癌症六大特征之一，在过去二十年中已得到广泛研究。我们研究的目的是评估喉癌中的微血管和巨噬细胞密度及其临床病理相关性。我们使用微阵列技术通过免疫组织化学评估微血管密度（CD34）和巨噬细胞计数（CD68），然后寻找临床病理相关性。研究组在×200视野中的平均微血管密度为14.27±12.92个血管，平均巨噬细胞浸润密度为5.19±4.32。有转移患者的微血管密度中位数显著高于无转移患者。此外，线性回归表明巨噬细胞浸润密度可预测喉癌中的微血管密度。我们发现这两个因素与复发率或其他临床特征之间均无关联。我们的研究为过去二十年中已被广泛研究的一个问题增添了更多数据，即使该领域仍存在争议。
