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大肠杆菌野生型和ada突变体中ogt基因的表达

Expression of the ogt gene in wild-type and ada mutants of E. coli.

作者信息

Potter P M, Kleibl K, Cawkwell L, Margison G P

机构信息

Department of Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.

出版信息

Nucleic Acids Res. 1989 Oct 25;17(20):8047-60. doi: 10.1093/nar/17.20.8047.

DOI:10.1093/nar/17.20.8047
PMID:2682522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334946/
Abstract

O6-alkylguanine (O6-AlkG) DNA alkyltransferase (ATase) and alkylphosphotriester (AlkP) ATase activity have been quantitated individually in extracts of various E. coli strains by means of ATase specific DNA substrates. O6-AlkG ATase activity was higher than AlkP ATase activity in the wild-type strains F26, AB1157 and SB229 and in the ada- mutants PJ1, PJ3, PJ5 and PJ6 indicating a 5-70 times higher level of expression of the ogt gene than the ada gene. The ada- mutant strains BS23, BS73 and GW5352 expressed O6-AlkG ATase but not AlkP ATase activity indicating expression only of the ogt gene. Southern analysis of DNA from F26, BS23, BS73, PJ1 and GW5352 showed a consistent pattern of hybridisation to an ogt probe but not to an ada probe. Exposure of E. coli to adaptive doses of N-methyl-N-nitro-N-nitroso-guanidine (MeNNG) caused an increase in AlkP ATase activity in F26, AB1156, SB229, PJ1, PJ3, PJ5 and PJ6. O6-AlkG ATase activity also increased in F26, AB1157 and SB229 but decreased to almost undetectable levels in all other strains examined except PJ3 where it remained constant. MeNNG increased ada mRNA abundance in F26 but no ada mRNA was detected in BS23, BS73 or GW5352: there was no evidence for increased ogt mRNA in any of the strains examined. In a limited survey, other bacterial strains have been shown to possess an ogt-like ATase activity.

摘要

利用特异性针对DNA烷基转移酶（ATase）的DNA底物，已对多种大肠杆菌菌株提取物中的O6-烷基鸟嘌呤（O6-AlkG）DNA烷基转移酶和烷基磷酸三酯（AlkP）ATase活性进行了单独定量。在野生型菌株F26、AB1157和SB229以及ada-突变体PJ1、PJ3、PJ5和PJ6中，O6-AlkG ATase活性高于AlkP ATase活性，这表明ogt基因的表达水平比ada基因高5至70倍。ada-突变体菌株BS23、BS73和GW5352表达O6-AlkG ATase，但不表达AlkP ATase活性，这表明仅表达ogt基因。对来自F26、BS23、BS73、PJ1和GW5352的DNA进行的Southern分析显示，与ogt探针杂交呈现一致模式，但与ada探针不杂交。用适应性剂量的N-甲基-N-硝基-N-亚硝基胍（MeNNG）处理大肠杆菌，导致F26、AB1156、SB229、PJ1、PJ3、PJ5和PJ6中的AlkP ATase活性增加。F26、AB1157和SB229中的O6-AlkG ATase活性也增加，但在所有其他检测菌株中降至几乎检测不到的水平，除了PJ3中其保持恒定。MeNNG增加了F26中ada mRNA的丰度，但在BS23、BS73或GW5352中未检测到ada mRNA：在所检测的任何菌株中均没有证据表明ogt mRNA增加。在一项有限的调查中，已证明其他细菌菌株具有类似ogt的ATase活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d38d/334946/01b944d3c40c/nar00137-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d38d/334946/60f4ecab3d95/nar00137-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d38d/334946/97292d8126d3/nar00137-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d38d/334946/01b944d3c40c/nar00137-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d38d/334946/60f4ecab3d95/nar00137-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d38d/334946/97292d8126d3/nar00137-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d38d/334946/01b944d3c40c/nar00137-0040-a.jpg

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本文引用的文献

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