Maaß Sandra, Becher Dörte
Institute for Microbiology, Ernst Moritz Arndt Universität Greifswald, D-17487 Greifswald, Germany.
Institute for Microbiology, Ernst Moritz Arndt Universität Greifswald, D-17487 Greifswald, Germany.
J Proteomics. 2016 Mar 16;136:222-33. doi: 10.1016/j.jprot.2016.01.015. Epub 2016 Jan 26.
In the last years the scientific community faced an increased need to provide high-quality data on the concentration of single proteins within a cell. Especially against the background of the fast evolving field of systems biology this does not only apply for a few proteins but preferably for the whole proteome of the organism. Therefore there has been a rapid development from pure identification of proteins via characterization of changes between different conditions by relative protein quantification towards determination of absolute protein amounts for hundreds of protein species in a cell. This review aims for discussion of different small-scale and large-scale approaches for absolute protein quantification in bacterial cells to picture biological processes and explore life in deeper detail. The presented advantages and limitations of various methods may provide interested researchers help to appraise available methods, select the most appropriate technique and avoid common pitfalls during determination of protein concentration in a complex sample.
在过去几年中，科学界面临着越来越大的需求，即要提供关于细胞内单个蛋白质浓度的高质量数据。特别是在系统生物学快速发展的背景下，这不仅适用于少数几种蛋白质，更适用于生物体的整个蛋白质组。因此，从通过相对蛋白质定量来表征不同条件之间的变化以单纯鉴定蛋白质，到确定细胞中数百种蛋白质的绝对含量，已经有了快速的发展。本综述旨在讨论用于细菌细胞中绝对蛋白质定量的不同小规模和大规模方法，以便更详细地描绘生物过程并探索生命。各种方法所呈现的优点和局限性可能会帮助感兴趣的研究人员评估现有方法，选择最合适的技术，并在复杂样品中蛋白质浓度测定过程中避免常见的陷阱。
