Wen Jing, Liu Qian-Wen, Luo Kong-Jia, Ling Yi-Hong, Xie Xiu-Ying, Yang Hong, Hu Yi, Fu Jian-Hua
State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, China.
Guangdong Esophageal Cancer Institute, 651 Dongfeng East Road, Guangzhou, 510060, China.
Tumour Biol. 2016 Aug;37(8):10141-8. doi: 10.1007/s13277-015-4633-2. Epub 2016 Jan 29.
The migration and invasion inhibitory protein (MIIP) was shown to function as a tumor suppressor gene in gliomas by inhibiting tumor cell growth, migration, and invasion. However, its role and clinical significance in esophageal squamous cell carcinoma (ESCC) have not been elucidated. We investigated the correlation of MIIP expression and clinical outcome in a group of surgically resected ESCCs. Tissue microarrays constructed of 253 surgically resected ESCC primary tumors and paired paracancerous normal esophageal epithelia were used for MIIP evaluation by immunohistochemistry. The clinical and prognostic significance of MIIP expression was analyzed statistically. The expression of MIIP expression in cancer tissues was increased significantly in comparison with the paired paracancerous normal epithelia (P < 0.001). And, MIIP expression was associated with ESCC cells' differentiation (P < 0.001). By Kaplan-Meier analysis, patients with low MIIP expression exhibited significantly improved overall survival (OS, P = 0.039) and a tendency of improved disease-free survival (DFS, P = 0.086) than those with high MIIP expression. In addition, MIIP expression could distinguish OS or DFS of patients with tumors in stage T3-4 (P = 0.020, 0.028), N0 (P = 0.008, 0.032), and stage II (P = 0.004, 0.019), as well as at lower thoracic esophagus (P = 0.024, 0.090). Multivariate analysis showed that MIIP expression was an independent prognostic factor in ESCC OS and DFS. In conclusion, MIIP expressed higher in ESCCs than in paracancerous normal esophageal epithelia and was a positive, independent prognostic factor in resected ESCCs.
迁移和侵袭抑制蛋白（MIIP）已被证明通过抑制肿瘤细胞生长、迁移和侵袭，在胶质瘤中发挥肿瘤抑制基因的作用。然而，其在食管鳞状细胞癌（ESCC）中的作用和临床意义尚未阐明。我们研究了一组手术切除的ESCC中MIIP表达与临床结果的相关性。由253例手术切除的ESCC原发性肿瘤和配对的癌旁正常食管上皮构建的组织芯片用于通过免疫组织化学评估MIIP。对MIIP表达的临床和预后意义进行统计学分析。与配对的癌旁正常上皮相比，癌组织中MIIP表达显著增加（P < 0.001）。并且，MIIP表达与ESCC细胞分化相关（P < 0.001）。通过Kaplan-Meier分析，MIIP低表达患者的总生存期（OS，P = 0.039）显著改善，无病生存期（DFS，P = 0.086）有改善趋势，高于MIIP高表达患者。此外，MIIP表达可以区分T3-4期（P = 0.020，0.028）、N0期（P = 0.008，0.032）和II期（P = 0.004，0.019）以及食管下段（P = 0.024，0.090）肿瘤患者的OS或DFS。多因素分析表明，MIIP表达是ESCC OS和DFS的独立预后因素。总之，MIIP在ESCC中的表达高于癌旁正常食管上皮，是切除的ESCC中的一个积极的独立预后因素。
