Shi Fei, Zhao Tian-Zhi, Wang Yong-Chun, Cao Xin-Sheng, Yang Chang-Bin, Gao Yuan, Li Cheng-Fei, Zhao Jiang-Dong, Zhang Shu, Sun Xi-Qing
Department of Aerospace Biodynamics, Faculty of Aerospace Medicine, The Fourth Military Medical University, Xi'an, China.
Cell Physiol Biochem. 2016;38(2):502-13. doi: 10.1159/000438646. Epub 2016 Feb 1.
BACKGROUND/AIMS: The potential role of caveolin-1 in modulating angiogenesis in microgravity environment is unexplored.
Using simulated microgravity by clinostat, we measured the expressions and interactions of caveolin-1 and eNOS in human umbilical vein endothelial cells.
We found that decreased caveolin-1 expression is associated with increased expression and phosphorylation levels of eNOS in endothelial cells stimulated by microgravity, which causes a dissociation of eNOS from caveolin-1 complexes. As a result, microgravity induces cell migration and tube formation in endothelial cell in vitro that depends on the regulations of caveolin-1.
Our study provides insight for the important endothelial functions in altered gravitational environments.
背景/目的：小窝蛋白-1在微重力环境中调节血管生成的潜在作用尚未得到探索。
通过回转器模拟微重力，我们测量了人脐静脉内皮细胞中小窝蛋白-1和内皮型一氧化氮合酶（eNOS）的表达及相互作用。
我们发现，在微重力刺激的内皮细胞中，小窝蛋白-1表达降低与eNOS表达增加及磷酸化水平升高相关，这导致eNOS从小窝蛋白-1复合物中解离。结果，微重力在体外诱导内皮细胞迁移和管腔形成，这依赖于小窝蛋白-1的调节。
我们的研究为重力环境改变时重要的内皮功能提供了见解。
