Kim Daehan, Nguyen Que Thanh Thanh, Lee Seungjin, Choi Kyung-Mi, Lee Eun-Ju, Park Joong Yull
Department of Mechanical Engineering, Graduate School, Chung-Ang University, Seoul, 06974, Republic of Korea.
Department of Obstetrics and Gynecology, School of Medicine, Chung-Ang University, Seoul, 06974, Republic of Korea.
NPJ Microgravity. 2023 Aug 11;9(1):63. doi: 10.1038/s41526-023-00311-1.
Over the past few decades, research on life in space has increased. Owing to the expensive nature of and the challenges associated with conducting experiments in real space, clinostats, which continuously randomize the gravity vector by using motors, have been used to generate simulated microgravity (SMG) on Earth. Herein, by using a 3D printing method, we develop a customized small-sized clinostat (CS clinostat) that is easy to manufacture, inexpensive, and robust. Moreover, we develop and fabricate a gas-permeable polydimethylsiloxane culture dish that fits inside the CS clinostat. To validate SMG generation, ovarian cancer cells (OV- 90, TOV-21G, and Caov-3) were applied to demonstrate a significant reduction in caveolin-1 expression, a biomarker of SMG, indicating SMG generation. The proposed CS clinostat system has good accessibility for SMG research, which makes it useful as a tool for biologists, who are unfamiliar with conventional clinostat equipment, to conduct preliminary studies in the space environment.
在过去几十年里，对太空生命的研究有所增加。由于在实际太空进行实验成本高昂且面临诸多挑战，利用电机持续随机化重力矢量的回转器已被用于在地球上产生模拟微重力（SMG）。在此，我们通过3D打印方法开发了一种定制的小型回转器（CS回转器），它易于制造、成本低廉且坚固耐用。此外，我们还开发并制造了一种适合CS回转器内部的透气聚二甲基硅氧烷培养皿。为验证模拟微重力的产生，我们应用卵巢癌细胞（OV - 90、TOV - 21G和Caov - 3）来证明作为模拟微重力生物标志物的小窝蛋白-1表达显著降低，这表明产生了模拟微重力。所提出的CS回转器系统为模拟微重力研究提供了良好的便利性，这使其成为生物学家进行太空环境初步研究的有用工具，这些生物学家并不熟悉传统的回转器设备。
