Ho Jeff C H, Hsiao C D, Kawakami K, Tse William K F
Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, Taiwan.
Aquat Toxicol. 2016 Apr;173:29-35. doi: 10.1016/j.aquatox.2016.01.001. Epub 2016 Jan 13.
Triclosan (TCS) is an active antimicrobial ingredient used in many household products, such as skin creams and toothpaste. It is produced in high volumes, and humans are directly exposed to it and dispose it on a daily basis. TCS has been found to contaminate water worldwide. This study aimed to understand the potential developmental and metabolic abnormalities caused by TCS exposure by using zebrafish as the experimental model. Four developmental stages (70-85% epiboly, 10-12 somite, prim-5, and 5dpf) were selected to perform in situ hybridization staining to investigate the effects of TCS on dorsal ventral patterning, segmentation, brain development, and organ formation. Results showed, in terms of developmental toxicology, that neither phenotypic nor molecular changes were found after 5 days of 250μg/L TCS exposure. However, such dosage of TCS exposure resulted in lipid droplet accumulation in the yolk sac, which might due to the deregulated mRNA expression level of beta-oxidation transcripts. This study showed that 250μg/L TCS exposure does not affect normal embryogenesis or organogenesis; however, there are concerns regarding possible impairment of lipid metabolism.
三氯生（TCS）是一种活性抗菌成分，用于许多家用产品，如护肤霜和牙膏。它的产量很高，人类每天都会直接接触并使用它。已发现TCS在全球范围内污染水源。本研究旨在以斑马鱼为实验模型，了解TCS暴露可能导致的发育和代谢异常。选择了四个发育阶段（胚盘外包70 - 85%、10 - 12体节、prim - 5和5天胚胎期）进行原位杂交染色，以研究TCS对背腹模式形成、体节形成、脑发育和器官形成的影响。结果表明，从发育毒理学角度来看，250μg/L TCS暴露5天后，未发现表型或分子变化。然而，这种剂量的TCS暴露导致卵黄囊中脂滴积累，这可能是由于β -氧化转录本的mRNA表达水平失调所致。本研究表明，250μg/L TCS暴露不会影响正常胚胎发生或器官发生；然而，脂质代谢可能受损仍令人担忧。
