Fromm Steffanie, Senkler Jennifer, Zabaleta Eduardo, Peterhänsel Christoph, Braun Hans-Peter
Institut für Pflanzengenetik, Leibniz Universität Hannover, Hannover, 30419, Germany.
Institut für Botanik, Leibniz Universität Hannover, Hannover, 30419, Germany.
Physiol Plant. 2016 Jul;157(3):289-96. doi: 10.1111/ppl.12424. Epub 2016 Apr 5.
The mitochondrial NADH dehydrogenase complex (complex I) consists of several functional domains which independently arose during evolution. In higher plants, it contains an additional domain which includes proteins resembling gamma-type carbonic anhydrases. The Arabidopsis genome codes for five complex I-integrated gamma-type carbonic anhydrases (γCA1, γCA2, γCA3, γCAL1, γCAL2), but only three copies of this group of proteins form an individual extra domain. Biochemical analyses revealed that the domain is composed of one copy of either γCAL1 or γCAL2 plus two copies of the γCA1/γCA2 proteins. Thus, the carbonic anhydrase domain can have six distinct subunit configurations. Single and double mutants with respect to the γCA/γCAL proteins were employed to genetically dissect the function of the domain. New insights into complex I biology in plants will be reviewed and discussed.
线粒体NADH脱氢酶复合体（复合体I）由几个在进化过程中独立出现的功能结构域组成。在高等植物中，它包含一个额外的结构域，其中包括类似于γ型碳酸酐酶的蛋白质。拟南芥基因组编码五种整合到复合体I中的γ型碳酸酐酶（γCA1、γCA2、γCA3、γCAL1、γCAL2），但这一组蛋白质中只有三个拷贝形成一个单独的额外结构域。生化分析表明，该结构域由γCAL1或γCAL2的一个拷贝加上γCA1/γCA2蛋白质的两个拷贝组成。因此，碳酸酐酶结构域可以有六种不同的亚基构型。利用γCA/γCAL蛋白的单突变体和双突变体对该结构域的功能进行了遗传学剖析。将对植物复合体I生物学的新见解进行综述和讨论。
