Souza A L P, Lima G L, Peixoto G C X, Silva A M, Oliveira M F, Silva A R
Laboratory of Animal Germplasm Conservation-LCGA, Departmento of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil.
Laboratory of Animal Germplasm Conservation-LCGA, Departmento of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil.
Theriogenology. 2016 May;85(8):1432-8. doi: 10.1016/j.theriogenology.2016.01.007. Epub 2016 Jan 12.
As an alternative for the conservation of collared peccary semen, this research aims at evaluating the use of Aloe vera (AV) extract as a cryoprotectant for semen chilling and freezing. Five ejaculates were divided in two aliquots that were diluted in Tris plus egg yolk (EY; 20%) or AV extract (20%) and chilled at 5 °C. In both treatments, an adequate semen conservation was achieved and values closer to 40% motile sperm with viability and osmotic response ranging from 20% to 40%, and normal morphology of 80% were found after 36 hours of storage. Moreover, 12 other ejaculates were diluted in Tris plus EY (20%) or AV extract (5, 10, or 20%) and glycerol (3%). Samples were frozen in liquid nitrogen and thawed after 1 week. After thawing, all the treatments containing EY or AV provided similar values for sperm morphology, viability, osmotic response, membrane integrity, sperm motility, amplitude of lateral head, beat cross frequency, and rapid, low, and static subpopulations, but the highest values for straightness and the lowest values for curvilinear velocity were found using 20% AV (P < 0.05). In conclusion, we found that AV extract at a 20% concentration could be used as an alternative substitute to EY in the formulation of Tris extenders for collared peccaries' semen chilling or freezing.
作为野猪精液保存的一种替代方法，本研究旨在评估使用芦荟（AV）提取物作为精液冷藏和冷冻的冷冻保护剂。将5份射精样本分成两份等分试样，分别用Tris加蛋黄（EY；20%）或AV提取物（20%）稀释，并在5℃下冷藏。在两种处理中，精液均得到了充分保存，储存36小时后，发现活动精子值接近40%，活力和渗透反应在20%至40%之间，正常形态率为80%。此外，将另外12份射精样本用Tris加EY（20%）或AV提取物（5%、10%或20%）以及甘油（3%）稀释。样本在液氮中冷冻，并在1周后解冻。解冻后，所有含EY或AV的处理在精子形态、活力、渗透反应、膜完整性、精子活力、头部侧向摆动幅度、拍频交叉频率以及快速、低速和静态亚群方面提供了相似的值，但使用20% AV时直线度最高，曲线速度最低（P < 0.05）。总之，我们发现20%浓度的AV提取物可作为Tris稀释剂配方中EY的替代物，用于野猪精液的冷藏或冷冻。
