Santos Caio Sérgio, Cavalcante Yasmim Carla da Silva, Campos Lívia Batista, da Silva Andréia Maria, Feijó Francisco Marlon Carneiro, Silva Alexandre Rodrigues
Laboratório de Conservação de Germoplasma Animal, Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido - UFERSA, Mossoró, RN, Brasil.
Laboratório de Microbiologia e Virologia, Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido - UFERSA, Mossoró RN, Brasil.
Anim Reprod. 2024 Aug 5;21(3):e20240018. doi: 10.1590/1984-3143-AR2024-0018. eCollection 2024.
The effects of antibiotics on sperm longevity in collared peccary () fresh diluted semen was evaluated. Semen samples from six adult males were collected by electroejaculation and diluted in Tris-citrate-fructose alone (control) and plus streptomycin-penicillin (2 mg/ml-2000 IU/ml) or gentamicin (70 µg/ml). Membrane integrity and functionality, mitochondrial activity and sperm morphology were assessed subjectively. Sperm motility and other kinetic parameters were objectively assessed using CASA (computer-assisted semen analysis). The semen diluted according to the treatments were submitted to the thermoresistance test, incubated at 37 ° C, and the sperm parameters analyzed at 0, 30, 60, 120 and 180 min. The average values of the treatments were compared with each other and between the times. There were no differences (P > 0.05) between treatments until the end of the test. Control and streptomycin-penicillin samples maintained sperm function for up to 180 min (with total motility of 24.3 ± 7.1% and 28 ± 8.7%, respectively). Gentamicin aliquots retained most parameters until the end of the incubation, except for membrane integrity and mitochondrial activity that declined (P < 0.05) at 180 min (53.1 ± 7.1% and 50.7 ± 6.2%, respectively) compared to 0 min (80.5 ± 4.7% and 86.3 ± 3.4%, respectively). In conclusion, a multiparametric thermoresistance test proved that Tris-based extenders used for collared peccary semen can be effectively supplemented by streptomycin-penicillin (2 mg/ml-2000 IU/ml) or gentamicin (70 µg/ml), especially during 180-min incubation at 37 °C.
评估了抗生素对领西貒（）新鲜稀释精液中精子存活时间的影响。通过电刺激采得6只成年雄性的精液样本，并分别用单独的柠檬酸三钠 - 果糖（对照）以及添加链霉素 - 青霉素（2毫克/毫升 - 2000国际单位/毫升）或庆大霉素（70微克/毫升）进行稀释。主观评估精子膜完整性与功能、线粒体活性及精子形态。使用计算机辅助精液分析（CASA）客观评估精子活力及其他动力学参数。根据处理方法稀释后的精液进行耐热性测试，在37°C下孵育，并在0、30、60、120和180分钟时分析精子参数。比较各处理组之间以及不同时间点的平均值。直到测试结束，各处理组之间均无差异（P>0.05）。对照和链霉素 - 青霉素样本可使精子功能维持长达180分钟（总活力分别为24.3±7.1%和28±8.7%）。庆大霉素等分试样在孵育结束前保留了大部分参数，但膜完整性和线粒体活性在180分钟时下降（P<0.05）（分别为53.1±7.1%和50.7±6.2%），而在0分钟时分别为80.5±4.7%和86.3±3.4%。总之，多参数耐热性测试证明，用于领西貒精液的基于Tris的稀释剂可有效添加链霉素 - 青霉素（2毫克/毫升 - 2000国际单位/毫升）或庆大霉素（70微克/毫升），尤其是在37°C下孵育180分钟期间。
