使用多散斑扩散波谱法分析急性大鼠脑切片中去相关时间与组织厚度之间的关系。
Analyzing the relationship between decorrelation time and tissue thickness in acute rat brain slices using multispeckle diffusing wave spectroscopy.
作者信息
Brake Joshua, Jang Mooseok, Yang Changhuei
出版信息
J Opt Soc Am A Opt Image Sci Vis. 2016 Feb 1;33(2):270-5. doi: 10.1364/JOSAA.33.000270.
Abstract
Novel techniques in the field of wavefront shaping have enabled light to be focused deep inside or through scattering media such as biological tissue. However, most of these demonstrations have been limited to thin, static samples since these techniques are very sensitive to changes in the arrangement of the scatterers within. As the samples of interest get thicker, the influence of the dynamic nature of the sample becomes even more pronounced and the window of time in which the wavefront solutions remain valid shrinks further. In this paper, we examine the time scales upon which this decorrelation happens in acute rat brain slices via multispeckle diffusing wave spectroscopy and investigate the relationship between this decorrelation time and the thickness of the sample using diffusing wave spectroscopy theory and Monte Carlo photon transport simulation.
摘要
波前整形领域的新技术使光线能够聚焦到生物组织等散射介质内部深处或穿透这些介质。然而，这些演示大多局限于薄的静态样本，因为这些技术对内部散射体排列的变化非常敏感。随着感兴趣的样本变厚，样本动态特性的影响变得更加显著，波前解决方案保持有效的时间窗口进一步缩小。在本文中，我们通过多散斑扩散波谱研究急性大鼠脑切片中这种去相关现象发生的时间尺度，并使用扩散波谱理论和蒙特卡罗光子输运模拟研究这种去相关时间与样本厚度之间的关系。
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