Kadeh Hamideh, Derakhshanfar Ghazal, Saravani Shirin
Department of Oral & Maxillofacial Pathology, Oral and Dental Disease Research Center, School of Dentistry, Zahedan University of Medical Science, ZAHEDAN, IRAN.
Turk Patoloji Derg. 2016;32(1):22-6. doi: 10.5146/tjpath.2015.01338.
Reactive hyperplastic lesions are one of the most common lesions of the oral cavity. Mast cells can be found in various oral lesions. However, the exact role of mast cells in oral reactive lesions is not fully understood. The aim of this study was to compare the mast cells count in various oral reactive lesions and to evaluate the correlation between the mast cells number and the intensity of inflammation.
This cross-sectional study was performed on 80 samples of oral reactive lesions including irritation fibroma, pyogenic granuloma, peripheral giant cell granuloma and peripheral ossifying fibroma (20 for each). Eight samples of normal oral mucosa were selected as the control group. To determine the mast cells count and the intensity of inflammation, Toluidine blue and Hematoxylin & Eosin staining was performed respectively. The data was analyzed with SPSS (V.21), Kruskal-Wallis, Tukey and Spearman's correlation coefficient tests.
In this study, a significant increase in mast cells count was found in oral reactive lesions compared to normal oral mucosa (p < 0.001). Difference of mast cell count between irritation fibroma and the peripheral giant cell granuloma groups (p=0.023), peripheral ossifying fibroma and peripheral giant cell granuloma groups (p=0.001) was statistically significant. There was a significant correlation between mast cell count and the intensity of inflammation in the irritation fibroma and pyogenic granuloma groups.
The finding of this study showed that mast cells count in peripheral ossifying fibroma and irritation fibroma were significantly higher than normal oral mucosa, so mast cells may have some role in the induction of fibrosis in oral reactive lesions.
反应性增生性病变是口腔最常见的病变之一。肥大细胞可见于各种口腔病变中。然而，肥大细胞在口腔反应性病变中的确切作用尚未完全明确。本研究旨在比较各种口腔反应性病变中的肥大细胞计数，并评估肥大细胞数量与炎症强度之间的相关性。
本横断面研究对80例口腔反应性病变样本进行，包括刺激性纤维瘤、化脓性肉芽肿、外周性巨细胞肉芽肿和外周性骨化性纤维瘤（各20例）。选取8例正常口腔黏膜样本作为对照组。分别采用甲苯胺蓝染色和苏木精-伊红染色来确定肥大细胞计数和炎症强度。数据采用SPSS（V.21）软件进行分析，采用Kruskal-Wallis检验、Tukey检验和Spearman相关系数检验。
本研究发现，与正常口腔黏膜相比，口腔反应性病变中的肥大细胞计数显著增加（p < 0.001）。刺激性纤维瘤组与外周性巨细胞肉芽肿组之间（p = 0.023）、外周性骨化性纤维瘤组与外周性巨细胞肉芽肿组之间（p = 0.001）的肥大细胞计数差异具有统计学意义。在刺激性纤维瘤和化脓性肉芽肿组中，肥大细胞计数与炎症强度之间存在显著相关性。
本研究结果表明，外周性骨化性纤维瘤和刺激性纤维瘤中的肥大细胞计数显著高于正常口腔黏膜，因此肥大细胞可能在口腔反应性病变的纤维化诱导中发挥一定作用。
