Mörschbächer P D, Alves Garcez T N, Paz A H, Magrisso A B, Mello H F, Rolim V M, Neuwald E B, Driemeier D, Contesini E A, Cirne-Lima E
Graduate Program in Veterinary Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9090, CEP 91540-000 Porto Alegre, Brazil; Laboratory of Embryology, Porto Alegre Clinical Hospital, Porto Alegre, Brazil.
Graduate Program in Veterinary Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9090, CEP 91540-000 Porto Alegre, Brazil.
Vet J. 2016 Mar;209:180-5. doi: 10.1016/j.tvjl.2015.11.009. Epub 2015 Nov 24.
Dilated cardiomyopathy (DCM) is a major cause of cardiovascular mortality and morbidity, and there is evidence to suggest that stem cell transplantation may be a viable treatment option for this condition. Therefore, the goal of the present study was to assess myocardial regeneration in rabbits with doxorubicin-induced DCM treated with adipose mesenchymal stem cells (MSC) alone or in combination with platelet-rich plasma (PRP). Twenty New Zealand rabbits received doxorubicin for the induction of DCM and were divided into four groups according to treatment: saline, MSC, PRP and MSC + RP. Treatment agents were injected directly into the left ventricular myocardium following a thoracoscopy. Rabbits were assessed through echocardiographic and electrocardiographic examinations, as well as serum cardiac troponin I measurements at baseline, after the induction of DCM and 15 days after treatment. Animals were euthanased following the last assessment, and hearts were collected for histopathological analyses. The MSC group showed improvements in all parameters assessed, while the PRP group showed significantly impaired heart function. Histopathology of the heart revealed that the MSC group displayed the lowest number of lesions, while rabbits in the MSC + PRP, saline and PRP groups had steadily advancing lesions. These results suggest that MSC transplantation can improve heart function in rabbits with DCM, and underscore the need for further studies of the effects of PRP on the myocardium.
扩张型心肌病（DCM）是心血管疾病死亡率和发病率的主要原因，有证据表明干细胞移植可能是治疗这种疾病的可行选择。因此，本研究的目的是评估单独使用脂肪间充质干细胞（MSC）或与富血小板血浆（PRP）联合治疗阿霉素诱导的DCM兔的心肌再生情况。20只新西兰兔接受阿霉素诱导DCM，并根据治疗方法分为四组：生理盐水组、MSC组、PRP组和MSC+PRP组。在胸腔镜检查后将治疗药物直接注射到左心室心肌中。在基线、诱导DCM后和治疗后15天，通过超声心动图和心电图检查以及血清心肌肌钙蛋白I测量对兔进行评估。在最后一次评估后对动物实施安乐死，并收集心脏进行组织病理学分析。MSC组在所有评估参数上均有改善，而PRP组心脏功能明显受损。心脏组织病理学显示，MSC组病变数量最少，而MSC+PRP组、生理盐水组和PRP组的兔病变逐渐加重。这些结果表明，MSC移植可以改善DCM兔的心脏功能，并强调需要进一步研究PRP对心肌的影响。
