Asamoto M, Fukushima S, Oosumi H, Tatemoto Y, Mori M
First Department of Pathology, Nagoya City University Medical School, Japan.
Urol Res. 1989;17(5):273-7. doi: 10.1007/BF00262981.
Expression of epithelial membrane antigen (EMA) was investigated immunohistochemically in 27 cases of bladder carcinoma using a monoclonal antibody. Normal urothelial epithelium showed EMA staining restricted to the upper layer of the surface epithelium. G-I transitional cell carcinomas demonstrated positive EMA staining which could be divided into the following 3 types; type 1, in which highly stained cells occurred in the upper layer of the neoplastic epithelium; type 2, in which the whole tumour focus was slightly stained; and type 3, in which cells strongly positive for EMA were scattered throughout the tumour focus. G-III (undifferentiated) transitional cell lesions exhibited irregular expression of EMA whereas squamous cell demonstrated specific intense EMA staining within keratinized tumour cells.
采用单克隆抗体，通过免疫组织化学方法对27例膀胱癌上皮膜抗原（EMA）的表达情况进行了研究。正常尿路上皮仅在表层上皮的上层显示EMA染色。G-I级移行细胞癌呈现EMA阳性染色，可分为以下3种类型：1型，肿瘤上皮上层出现高染色细胞；2型，整个肿瘤灶轻度染色；3型，EMA强阳性细胞散在于整个肿瘤灶。G-III级（未分化）移行细胞病变呈现EMA不规则表达，而鳞状细胞癌在角化的肿瘤细胞内显示特异性强EMA染色。
