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用于诊断免疫细胞化学的抗人上皮膜抗原单克隆抗体的制备。

Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.

作者信息

Cordell J, Richardson T C, Pulford K A, Ghosh A K, Gatter K C, Heyderman E, Mason D Y

出版信息

Br J Cancer. 1985 Sep;52(3):347-54. doi: 10.1038/bjc.1985.200.

DOI:10.1038/bjc.1985.200
PMID:3899155
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1977193/
Abstract

Two monoclonal murine antibodies have been raised against a delipidated extract of human cream. These antibodies were detected by immunohistological screening of hybridoma culture supernatants on sections of human breast tissue. One of those antibodies (E29) was subsequently screened against a range of normal and neoplastic human tissues and shown to react with a wide variety of human epithelia and with mesothelial cells. Antibody E29 was unreactive with other cell types, with the exception of occasional plasma cells. Antibody E29 is suitable for use on paraffin embedded tissue and represents a valuable reagent for the identification of tumours of epithelial origin.

摘要

已制备出两种针对人乳脱脂提取物的鼠单克隆抗体。通过在人乳腺组织切片上对杂交瘤培养上清液进行免疫组织学筛选来检测这些抗体。随后，其中一种抗体（E29）针对一系列正常和肿瘤性人类组织进行筛选，结果显示它能与多种人类上皮细胞和间皮细胞发生反应。抗体E29除偶尔与浆细胞反应外，与其他细胞类型无反应。抗体E29适用于石蜡包埋组织，是鉴定上皮源性肿瘤的一种有价值的试剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/d0fa1d415429/brjcancer00095-0060-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/e160e6eeb1bd/brjcancer00095-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/6f47318a72a2/brjcancer00095-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/ce70a26ebf7e/brjcancer00095-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/82627350df9c/brjcancer00095-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/e988c61c5d2a/brjcancer00095-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/fc15d23fce58/brjcancer00095-0060-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/d0fa1d415429/brjcancer00095-0060-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/e160e6eeb1bd/brjcancer00095-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/6f47318a72a2/brjcancer00095-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/ce70a26ebf7e/brjcancer00095-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/82627350df9c/brjcancer00095-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/e988c61c5d2a/brjcancer00095-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/fc15d23fce58/brjcancer00095-0060-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3baf/1977193/d0fa1d415429/brjcancer00095-0060-d.jpg

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本文引用的文献

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An immunocytochemical study of the distribution of epithelial membrane antigen in normal and disordered squamous epithelium.一项关于上皮膜抗原在正常及病变鳞状上皮中分布的免疫细胞化学研究。
Diagn Histopathol. 1982 Jan-Mar;5(1):11-7.
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Use of antisera to epithelial membrane antigen for the cytodiagnosis of malignancy in serous effusions.使用抗上皮膜抗原血清进行浆液性积液中恶性肿瘤的细胞诊断。
J Clin Pathol. 1981 Dec;34(12):1326-32. doi: 10.1136/jcp.34.12.1326.
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The use of antisera to epithelial membrane antigen in detecting micrometastases in histological sections.
通过基于孔的反相蛋白质阵列评估一组肿瘤标志物用于鉴别诊断良性和恶性积液。
Diagn Pathol. 2015 May 29;10:53. doi: 10.1186/s13000-015-0290-4.
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Stem cell marker TRA-1-60 is expressed in foetal and adult kidney and upregulated in tubulo-interstitial disease.干细胞标志物 TRA-1-60 在胎儿和成人肾脏中表达，并在上皮间质疾病中上调。
Histochem Cell Biol. 2010 Oct;134(4):355-69. doi: 10.1007/s00418-010-0741-7. Epub 2010 Sep 19.
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KL-6 is another useful marker in assessing a micropapillary pattern in carcinomas of the breast and urinary bladder, but not the colon.KL-6是评估乳腺癌和膀胱癌微乳头模式的另一个有用标志物，但不是结肠癌的。
Med Mol Morphol. 2009 Jun;42(2):123-7. doi: 10.1007/s00795-009-0450-2. Epub 2009 Jun 18.
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Overexpression and altered glycosylation of MUC1 in malignant mesothelioma.恶性间皮瘤中MUC1的过表达及糖基化改变
Br J Cancer. 2008 May 6;98(9):1562-9. doi: 10.1038/sj.bjc.6604340. Epub 2008 Apr 29.
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Immunohistochemical and immunoelectron microscopic studies of the localization of KL-6 and epithelial membrane antigen (EMA) in presumably normal pulmonary tissue and in interstitial pneumonia.KL-6和上皮膜抗原（EMA）在疑似正常肺组织和间质性肺炎中的定位的免疫组织化学和免疫电子显微镜研究。
Med Mol Morphol. 2007 Dec;40(4):198-202. doi: 10.1007/s00795-007-0382-7. Epub 2007 Dec 21.
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MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.MUC1（上皮膜抗原）在ALK阳性间变性大细胞淋巴瘤中优先以正常糖基化或仅部分低聚糖基化的形式表达。
J Clin Pathol. 2001 Dec;54(12):933-9. doi: 10.1136/jcp.54.12.933.
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[Tumor cell dissemination in bone marrow and peritoneal cavity. An immunocytochemical study of patients with stomach or colorectal carcinoma].[肿瘤细胞在骨髓和腹腔中的播散。对胃癌或结直肠癌患者的免疫细胞化学研究]
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