Shi Yan-Hong, Zhu Shu, Tamura Takayuki, Kadowaki Makoto, Wang Zhengtao, Yoshimatsu Kayo, Komatsu Katsuko
Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan.
Toyama Prefectural Medicinal Plants Center, Toyama, 930-0412, Japan.
J Nat Med. 2016 Apr;70(2):234-40. doi: 10.1007/s11418-016-0966-4. Epub 2016 Jan 30.
The methanolic extract and its subfractions from the dried root of Edulis Superba, a horticultural cultivar of Paeonia lactiflora Pallas, showed potent anti-allergic effect as inhibition of immunoglobulin E (IgE)-mediated degranulation in rat basophil leukemia (RBL)-2H3 cells. Bioassay-guided fractionation led to the isolation of 26 compounds, including a new norneolignan glycoside, paeonibenzofuran (1), together with 25 known ones (2-26). The chemical structure of the new compound was elucidated on the basis of spectroscopic and chemical evidences. Among the isolated compounds, mudanpioside E (5) with paeoniflorin-type skeleton and quercetin (16) showed potent inhibitory activity against a degranulation marker, β-hexosaminidase release with IC50 values of 40.34 and 25.05 μM, respectively. A primary structure-activity relationship of these components was discussed.
芍药栽培品种美丽芍药干燥根的甲醇提取物及其亚组分，对大鼠嗜碱性粒细胞白血病（RBL）-2H3细胞中免疫球蛋白E（IgE）介导的脱颗粒具有抑制作用，显示出强大的抗过敏作用。生物活性导向分离得到26种化合物，包括一种新的降新木脂素糖苷芍药苯并呋喃（1）以及25种已知化合物（2 - 26）。根据光谱和化学证据阐明了新化合物的化学结构。在分离得到的化合物中，具有芍药苷型骨架的牡丹皮苷E（5）和槲皮素（16）对脱颗粒标志物β-己糖胺酶释放显示出强大的抑制活性，IC50值分别为40.34和25.05 μM。讨论了这些成分的初步构效关系。
