Momota Yutaka, Shimada Kenichiro, Noguchi Azusa, Saito Akio, Nozawa Satoshi, Niina Ayaka, Tani Kenji, Azakami Daigo, Ishioka Katsumi, Sako Toshinori
School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
Laboratory of Biomolecular Chemistry, School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
Vet Dermatol. 2016 Apr;27(2):67-e19. doi: 10.1111/vde.12287. Epub 2016 Feb 2.
Corneocyte surface area (CSA) is as established parameter for skin barrier function in humans. Measurement of canine CSA has been previously reported but has not been validated.
HYPOTHESIS/OBJECTIVES: The aim of this study was to evaluate the validity of CSA as a barrier function parameter in dogs.
Six clinically normal beagle dogs.
CSA was measured and compared with transepidermal water loss (TEWL) using sequential tape stripping of the stratum corneum as a model of acute barrier disruption. Then, CSA and TEWL were measured at four anatomical sites (groin, lower back, nasal bridge and pinna). The correlation between the two indices was also evaluated.
From the results of sequential tape stripping, CSA values gradually decreased with increasing number of tape strippings. The CSA values were inversely correlated with the TEWL ones. The two indices at different sites were variable and were strongly correlated.
Canine CSA was demonstrated to be a useful parameter for the canine skin barrier function. The results from the anatomical sites imply that the cephalic sites (nasal bridge and pinna) were lower than others in skin barrier function.
角质形成细胞表面积（CSA）是评估人类皮肤屏障功能的既定参数。此前已有关于犬类CSA测量的报道，但尚未得到验证。
假设/目的：本研究旨在评估CSA作为犬类屏障功能参数的有效性。
六只临床健康的比格犬。
采用角质层连续胶带剥离法作为急性屏障破坏模型，测量CSA并与经表皮水分流失（TEWL）进行比较。然后，在四个解剖部位（腹股沟、下背部、鼻梁和耳廓）测量CSA和TEWL。同时评估这两个指标之间的相关性。
连续胶带剥离结果显示，CSA值随胶带剥离次数增加而逐渐降低。CSA值与TEWL值呈负相关。不同部位的这两个指标存在差异且相关性很强。
犬类CSA被证明是评估犬类皮肤屏障功能的有用参数。不同解剖部位的结果表明，头部部位（鼻梁和耳廓）的皮肤屏障功能低于其他部位。
