Oh W-S, Oh T-H
Hwanggum Animal Medical Centre, Daegu, Republic of Korea.
Aust Vet J. 2009 Oct;87(10):409-12. doi: 10.1111/j.1751-0813.2009.00481.x.
To establish a standardised clipping method for the measurement of transepidermal water loss (TEWL) with a VapoMeter in Beagle dogs and to identify the optimal anatomical site for TEWL measurement.
TEWL values obtained from skin sites on five healthy Beagles clipped using two different blade angles (standard vs non-standard) were compared. TEWL values for 48 h were also obtained from seven different anatomical sites that had differing hair density. The hair was clipped in the intensively haired anatomical sites (head, lower and upper back and tail), but not clipped in the sparsely haired sites (ear, inguinal region, footpad).
The TEWL values for the standard and non-standard clipping sites were 6.3 +/- 1.31 and 27.2 +/- 1.11 g/h/m(2), respectively. We found the upper back among the clipped sites was the most appropriate site for TEWL measurement over 48 h after clipping, whereas among the unclipped sites the ear was the most appropriate, because the TEWL values from those anatomical sites had the least fluctuation and were less affected by movement.
The clipping method and anatomical site should be standardised in order to minimise the experimental variation in TEWL measurement in dogs.
建立一种标准化的剪毛方法，用于使用VapoMeter测量比格犬的经表皮水分流失（TEWL），并确定TEWL测量的最佳解剖部位。
比较了五只健康比格犬使用两种不同刀片角度（标准与非标准）剪毛的皮肤部位获得的TEWL值。还从七个不同毛发密度的解剖部位获得了48小时的TEWL值。在毛发浓密的解剖部位（头部、背部上下和尾巴）剪毛，但在毛发稀疏的部位（耳朵、腹股沟区域、脚垫）不剪毛。
标准剪毛部位和非标准剪毛部位的TEWL值分别为6.3±1.31和27.2±1.11 g/h/m²。我们发现，在剪毛后的48小时内，剪毛部位中背部上方是TEWL测量的最合适部位，而在未剪毛部位中，耳朵是最合适的，因为这些解剖部位的TEWL值波动最小，受运动影响也较小。
应标准化剪毛方法和解剖部位，以尽量减少犬类TEWL测量中的实验差异。
