槲皮素对人类衰老过程中红细胞膜钠氢交换器的调节作用：与ATP酶的相关性
Quercetin-modulated erythrocyte membrane sodium-hydrogen exchanger during human aging: correlation with ATPase's.
作者信息
Kumar Prabhanshu, Chand Subhash, Maurya Pawan Kumar
机构信息
a Amity Institute of Biotechnology, Amity University Uttar Pradesh , Noida , India .
b Department of Biochemical Engineering & Biotechnology , Indian Institute of Technology , Delhi , India , and.
出版信息
Arch Physiol Biochem. 2016 Jul;122(3):141-7. doi: 10.3109/13813455.2016.1150299. Epub 2016 Feb 25.
CONTENT
Quercetin uptake by erythrocytes is rapid. The sodium-hydrogen exchanger (NHE) is a secondary active transporter, regulating intracellular pH, Na(+) concentration and cell volume.
OBJECTIVE
The aim of present study was to investigate NHE as a function of human age and effect of quercetin on its activity. The NHE activity was correlated with erythrocytes ATPases.
MATERIALS AND METHODS
We analyzed normal, healthy subjects of both sexes (20-82 years). NHE activity was estimated in terms of amiloride-sensitive H+-efflux from acid-loaded cells.
RESULTS
A significant age-dependent increase in NHE activity was observed during aging in humans. Concentration (10(-3 )M to 10(-8 )M)-dependent in vitro treatment with quercetin causes inhibition of NHE activity. The Na(+)/K(+) -ATPase (r = 0.8882) and Ca(2+)-ATPase (r = 0.9540) activities positively correlated with it.
DISCUSSION AND CONCLUSION
The present data show an additional mechanism where dietary flavonoids may exerts beneficial effect during aging.
内容
红细胞对槲皮素的摄取迅速。钠氢交换体（NHE）是一种继发性主动转运体，可调节细胞内pH值、Na⁺浓度和细胞体积。
目的
本研究旨在探讨NHE作为人类年龄的函数以及槲皮素对其活性的影响。NHE活性与红细胞ATP酶相关。
材料与方法
我们分析了20至82岁的正常健康男女受试者。通过酸负荷细胞中amiloride敏感的H⁺外流来估计NHE活性。
结果
在人类衰老过程中观察到NHE活性随年龄显著增加。用槲皮素进行浓度（10⁻³ M至10⁻⁸ M）依赖性的体外处理会导致NHE活性受到抑制。Na⁺/K⁺ -ATP酶（r = 0.8882）和Ca²⁺ -ATP酶（r = 0.9540）活性与之呈正相关。
讨论与结论
目前的数据显示了饮食类黄酮在衰老过程中可能发挥有益作用的另一种机制。
Zotero 插件