Singh Sandeep, Pandey Kanti Bhooshan, Rizvi Syed Ibrahim
a Department of Biochemistry , University of Allahabad , Allahabad , India.
Arch Physiol Biochem. 2016 Oct;122(4):228-234. doi: 10.1080/13813455.2016.1190761. Epub 2016 May 31.
Alterations at the level of plasma membrane are reported to play an important role in cellular senescence. The present study was undertaken to correlate cellular senescence, membrane transport processes and organismal aging. To achieve this objective activities of membrane linked Na/K ATPase (NKA), Na/H exchanger (NHE) and correlation with membrane hydrxyperoxide level, sialic acid content and membrane protein oxidation was studied in density-gradient fractionated young and old erythrocytes from 4 and 24 month old Wistar rats. The results reveal that cellular aging within the tissue is associated with significant decrease in activities of NKA and NHE of senescent erythrocytes in comparison to younger cell population of same age group. The result shows that impaired ion homeostasis due to altered membrane transporters including functional and compositional changes may be one of the reasons responsible behind rat erythrocyte aging.
据报道，质膜水平的改变在细胞衰老中起重要作用。本研究旨在关联细胞衰老、膜转运过程和机体衰老。为实现这一目标，对来自4月龄和24月龄Wistar大鼠的密度梯度分级的年轻和衰老红细胞中膜结合的钠钾ATP酶（NKA）、钠氢交换体（NHE）的活性以及与膜过氧化氢水平、唾液酸含量和膜蛋白氧化的相关性进行了研究。结果显示，与同一年龄组的年轻细胞群体相比，组织内的细胞衰老与衰老红细胞中NKA和NHE的活性显著降低有关。结果表明，由于包括功能和成分变化在内的膜转运体改变导致的离子稳态受损可能是大鼠红细胞衰老背后的原因之一。
