Sakthinathan Subramanian, Lee Hsin Fang, Chen Shen-Ming, Tamizhdurai P
Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
J Colloid Interface Sci. 2016 Apr 15;468:120-127. doi: 10.1016/j.jcis.2016.01.014. Epub 2016 Jan 11.
In the present work, a reduced graphene oxide (RGO) supported manganese tetraphenylporphyrin (Mn-TPP) nanocomposite was electrochemically synthesized and used for the highly selective and sensitive detection of dopamine (DA). The nuclear magnetic resonance, scanning electron microscopy and elemental analysis were confirmed the successful formation of RGO/Mn-TPP nanocomposite. The prepared RGO/Mn-TPP nanocomposite modified electrode exhibited an enhanced electrochemical response to DA with less oxidation potential and enhanced response current. The electrochemical studies revealed that the oxidation of the DA at the composite electrode is a surface controlled process. The cyclic voltammetry, differential pulse voltammetry and amperometry methods were enable to detect DA. The working linear range of the electrode was observed from 0.3 to 188.8 μM, limit of detection was 8 nM and the sensitivity was 2.606 μA μM(-1) cm(-2). Here, the positively charged DA and negatively charged porphyrin modified RGO can accelerate the electrocatalysis of DA via electrostatic attraction, while the negatively charged ascorbic acid (AA) repulsed by the negatively charged electrode surface which supported for good selectivity. The good recovery results obtained for the determination of DA present in DA injection samples and human pathological sample further revealed the good practicality of RGO/Mn-TPP nanocomposite film modified electrode.
在本工作中，通过电化学合成制备了一种还原氧化石墨烯（RGO）负载的四苯基卟啉锰（Mn-TPP）纳米复合材料，并将其用于多巴胺（DA）的高选择性和高灵敏检测。核磁共振、扫描电子显微镜和元素分析证实了RGO/Mn-TPP纳米复合材料的成功制备。所制备的RGO/Mn-TPP纳米复合材料修饰电极对DA表现出增强的电化学响应，具有更低的氧化电位和增强的响应电流。电化学研究表明，在复合电极上DA的氧化是一个表面控制过程。循环伏安法、差分脉冲伏安法和安培法均可用于检测DA。该电极的工作线性范围为0.3至188.8 μM，检测限为8 nM，灵敏度为2.606 μA μM⁻¹ cm⁻²。在此，带正电荷的DA与带负电荷的卟啉修饰的RGO可通过静电吸引加速DA的电催化作用，而带负电荷的电极表面对带负电荷的抗坏血酸（AA）产生排斥作用，从而实现良好的选择性。对DA注射剂样品和人体病理样品中DA的测定所获得的良好回收率结果进一步表明了RGO/Mn-TPP纳米复合膜修饰电极具有良好的实用性。
