McInnes Steven J P, Michl Thomas D, Delalat Bahman, Al-Bataineh Sameer A, Coad Bryan R, Vasilev Krasimir, Griesser Hans J, Voelcker Nicolas H
ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia , Adelaide, South Australia 5001, Australia.
Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
ACS Appl Mater Interfaces. 2016 Feb;8(7):4467-76. doi: 10.1021/acsami.5b12433. Epub 2016 Feb 15.
Controlling the release kinetics from a drug carrier is crucial to maintain a drug's therapeutic window. We report the use of biodegradable porous silicon microparticles (pSi MPs) loaded with the anticancer drug camphothecin, followed by a plasma polymer overcoating using a loudspeaker plasma reactor. Homogenous "Teflon-like" coatings were achieved by tumbling the particles by playing AC/DC's song "Thunderstruck". The overcoating resulted in a markedly slower release of the cytotoxic drug, and this effect correlated positively with the plasma polymer coating times, ranging from 2-fold up to more than 100-fold. Ultimately, upon characterizing and verifying pSi MP production, loading, and coating with analytical methods such as time-of-flight secondary ion mass spectrometry, scanning electron microscopy, thermal gravimetry, water contact angle measurements, and fluorescence microscopy, human neuroblastoma cells were challenged with pSi MPs in an in vitro assay, revealing a significant time delay in cell death onset.
控制药物载体的释放动力学对于维持药物的治疗窗至关重要。我们报道了使用负载抗癌药物喜树碱的可生物降解多孔硅微粒（pSi MPs），随后使用扬声器等离子体反应器进行等离子体聚合物包覆。通过播放AC/DC乐队的歌曲《Thunderstruck》使颗粒翻滚，从而获得均匀的“类聚四氟乙烯”涂层。包覆导致细胞毒性药物的释放明显减慢，并且这种效果与等离子体聚合物涂层时间呈正相关，范围从2倍到100倍以上。最终，在用飞行时间二次离子质谱、扫描电子显微镜、热重分析、水接触角测量和荧光显微镜等分析方法对pSi MPs的制备、负载和包覆进行表征和验证后，在体外实验中用pSi MPs处理人神经母细胞瘤细胞，结果显示细胞死亡开始出现明显的时间延迟。
