Kong Kin Weng, Mat-Junit Sarni, Aminudin Norhaniza, Hassan Fouad Abdulrahman, Ismail Amin, Abdul Aziz Azlina
Department of Molecular Medicine, Faculty of Medicine, University of Malaya , Kuala Lumpur , Malaysia.
Institute of Biological Sciences, Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.
PeerJ. 2016 Jan 28;4:e1628. doi: 10.7717/peerj.1628. eCollection 2016.
Barringtonia racemosa is a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80%) at concentration less than 250 µg/ml and 500 µg/ml, respectively. BLE and BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H2O2-induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots of B. racemosa can be an alternative bioactive ingredient in the prevention of oxidative damage.
棋盘脚树是一种具有药用价值的热带植物。在本研究中，对该植物嫩枝的叶水提取物（BLE）和茎水提取物（BSE）保护HepG2细胞免受氧化损伤的能力进行了研究。使用高效液相色谱-二极管阵列检测法（HPLC-DAD）和电喷雾电离质谱法（ESI-MS）鉴定出了由没食子酸、鞣花酸、原儿茶酸、槲皮素和山奈酚组成的五种主要多酚化合物。细胞活力测定表明，BLE和BSE在浓度分别低于250μg/ml和500μg/ml时无细胞毒性（细胞活力>80%）。通过铁离子还原抗氧化能力（FRAP）测定法测得，BLE和BSE改善了细胞抗氧化状态，并保护HepG2细胞免受过氧化氢诱导的细胞毒性。提取物还抑制了HepG2细胞中的脂质过氧化以及活性氧的产生。在氧化应激期间，BLE和BSE还能抑制超氧化物歧化酶和过氧化氢酶的活性。棋盘脚树的嫩枝可作为预防氧化损伤的替代生物活性成分。
