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人牙周膜干细胞片层的牙周再生特性评估

Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets.

作者信息

Kim Joong-Hyun, Ko Seok-Yeong, Lee Justin Ho, Kim Deok-Ho, Yun Jeong-Ho

机构信息

Department of Periodontology, Chonbuk National University School of Dentistry and Institute of Oral Bioscience, Jeonju, Korea.

Department of Bioengineering, University of Washington, Seattle, WA, USA.

出版信息

J Periodontal Implant Sci. 2017 Dec;47(6):402-415. doi: 10.5051/jpis.2017.47.6.402. Epub 2017 Dec 31.

DOI:10.5051/jpis.2017.47.6.402
PMID:29333326
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5764766/
Abstract

PURPOSE

The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum.

METHODS

In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet.

RESULTS

The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets.

CONCLUSIONS

This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.

摘要

目的

本研究旨在确定使用热响应性基质制备的图案化人牙周膜干细胞（hPDLSC）片的效果。

方法

在本研究中，我们使用纳米拓扑线索制备图案化的hPDLSC片，以调节细胞片的排列。

结果

hPDLSCs在各种表面图案宽度上均能快速形成单层。与在平坦表面上生长的细胞片相比，在纳米图案化基质上的细胞附着和生长没有显著差异。然而，图案化的hPDLSC片在早期显示出比未图案化的细胞片更高的牙周膜形成相关基因表达。

结论

本实验证实，图案化细胞片在设计hPDLSC片方面提供了灵活性，并且这些干细胞片可能是牙周再生治疗应用的候选者。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/5b6a4e3ee381/jpis-47-402-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/f3035c2b5c80/jpis-47-402-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/b8a2fc85e863/jpis-47-402-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/74bddf13b5a0/jpis-47-402-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/b0b3ee55cad6/jpis-47-402-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/5b6a4e3ee381/jpis-47-402-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/f3035c2b5c80/jpis-47-402-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/b8a2fc85e863/jpis-47-402-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/74bddf13b5a0/jpis-47-402-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/b0b3ee55cad6/jpis-47-402-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3760/5764766/5b6a4e3ee381/jpis-47-402-g005.jpg

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Periodontal-Derived Mesenchymal Cell Sheets Promote Periodontal Regeneration in Inflammatory Microenvironment.牙周来源的间充质细胞片层在炎症微环境中促进牙周再生。
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