Experimental Therapeutics Laboratory, Sansom Institute and Hanson Institute, School of Pharmacy and Medical Science, University of South Australia, Adelaide, SA, 5000, Australia.
Mawson Institute, University of South Australia, Adelaide, SA, 5095, Australia.
Adv Healthc Mater. 2016 Apr 20;5(8):956-65. doi: 10.1002/adhm.201500845. Epub 2016 Feb 4.
Synthetic materials employed for enhancing, replacing, or restoring biological functionality may be compromised by the host immune responses that they evoke. Surface modification has attracted substantial attention as a tool to modulate the host response to synthetic materials; however, how surface nanotopography combined with chemistry affects immune effector cell responses is still poorly understood. To address this open question, a unique set of model surfaces with controlled surface nanotopography in the range of 16, 38, and 68 nm has been generated. Tailored outermost surface chemistry that was amine, carboxyl, or methyl group rich has been provided. The combinations of these properties yield 12 surface types that are subject to functional assays assessing key immune effector cells, namely, primary neutrophil and macrophage responses in vitro. The data demonstrate that surface nanotopography leads to enhanced matrix metalloproteinase-9 production from primary neutrophils, and a decrease in pro-inflammatory cytokine secretion from primary macrophages. Together, these results are the first to directly compare the immunomodulatory effects of the cooperative interplay between surface nanotopography and chemistry.
用于增强、替代或恢复生物功能的合成材料可能会受到宿主免疫反应的影响。表面修饰作为一种调节宿主对合成材料反应的工具引起了广泛关注；然而，表面纳米形貌与化学性质如何影响免疫效应细胞反应仍知之甚少。为了解决这个悬而未决的问题，已经产生了一系列独特的具有可控表面纳米形貌（范围为 16、38 和 68nm）的模型表面。提供了富含胺、羧基或甲基基团的定制最外层表面化学性质。这些特性的组合产生了 12 种表面类型，这些表面类型可用于功能测定，评估关键的免疫效应细胞，即体外原代中性粒细胞和巨噬细胞的反应。数据表明，表面纳米形貌导致原代中性粒细胞产生基质金属蛋白酶-9 的增加，以及原代巨噬细胞促炎细胞因子分泌的减少。总之，这些结果首次直接比较了表面纳米形貌与化学性质协同作用的免疫调节效应。
