Thalassotalea sediminis sp. nov., isolated from coastal sediment.

A novel Gram-stain negative, facultatively anaerobic, rod-shaped and yellowish-white-pigmented marine bacterium, designated strain N211(T), was isolated from marine sediment obtained off the coastal area of Weihai, China. The cells are approximately 0.4-0.9 × 1.8-3.5 μm in size and motile by means of a polar flagellum. The strain grows optimally at 33 °C, pH 7.5-8.5 and in the presence of 3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences indicated that strain N211(T) fell within the clade comprising the type strains of species of the genus Thalassotalea. Strain N211(T) was most closely related to Thalassotalea ganghwensis DSM 15355(T) (96.4 %) based on the 16S rRNA gene sequence, and shared 94.4-96.4 % similarity with type strains of all members of the genus Thalassotalea. The predominant isoprenoid quinone of strain N211(T) was identified as ubiquinone-8 (Q-8). The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. C17:1 w8c, summed feature 3 (C16:1 w7c and/or iso-C 15:0 2-OH) and C16:0 were found to be main cellular fatty acids. The G+C content of the genomic DNA is 39.1 mol%. It is evident from phenotypic data and phylogenetic inference that strain N211(T) represents a novel species of the genus Thalassotalea, for which the name T. sediminis sp. nov. is proposed. The type strain is N211(T) (=KCTC 42588(T) = MCCC 1H00116(T)).