对转化和体内噬菌体包装具有高度感受态的大肠杆菌recA缺失菌株。
Escherichia coli recA deletion strains that are highly competent for transformation and for in vivo phage packaging.
作者信息
Kurnit D M
机构信息
Howard Hughes Medical Institute, Department of Pediatrics, University of Michigan Medical Center, Ann Arbor 48109-0650.
出版信息
Gene. 1989 Oct 30;82(2):313-5. doi: 10.1016/0378-1119(89)90056-5.
PMID:2684784
Abstract
I describe the construction of a variety of Escherichia coli recA deletion strains designed to facilitate molecular cloning. These recA deletion strains permit the efficient cloning of foreign inserts carried in plasmid, phage, cosmid, phasmid (phage-plasmid hybrid) or phosmid (phage-cosmid hybrid) vectors.
摘要
我描述了多种大肠杆菌recA缺失菌株的构建，这些菌株旨在促进分子克隆。这些recA缺失菌株能够高效克隆携带于质粒、噬菌体、黏粒、噬菌粒（噬菌体 - 质粒杂交体）或磷质体（噬菌体 - 黏粒杂交体）载体中的外源插入片段。
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